[Histonet] Strange circles in IHC slides

[Greg Dobbin]

Hi V.
That my friend is the result of a air bubble on the section. If it had
been unremoved xylene the hematoxylin would have been absent as well.
Cheers.
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"I find that the harder I work, the 
more luck I seem to have."
- Thomas Jefferson


>>> "V. Neubert" <histonet.nospam <@t> vneubert.com> 4/2/2009 1:12:18 PM
>>>
Hello,

I really have some real links to real pictures of real relevance of the

real histonet list. Really promised.

http://img12.imageshack.us/img12/8513/ts0402162049.jpg 
http://img13.imageshack.us/img13/6514/ts0402162104.jpg 

So, has ever anyone experienced sth. like this?
My conjugate control (every step except the antibody) was fine, nothing

to be seen about DAB and no circles at all.

I used Shandon single-use coverplates, sterile buffer, fresh antibody 
aliquots. Any idea?

Thanks,

V. Neubert

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