[Histonet] glutaraldehyde perfusion

[Geoff McAuliffe]

Hi Boma:

Whether or not a saline or buffer flush was used before glut. fixation 
is of little consequence to the effects of glut as a fixative.The 
saline/buffer washout is just to remove blood. In my experience most 
people overdo the washout.
I am curious as to what "disrupt the blood-brain barrier" means. The bbb 
is a very complex entity with both physical and metabolic components.I 
suspect the person who told you this is not well informed.

Geoff

Boma Fubara wrote:
> Can someone explain to me the mechanism by which glutaraldehyde would 
> disrupt the blood brain barrier if the brain had not been flushed prior to 
> fixation?
>
> Boma.
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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