[Histonet] Help with McDowell and Trump's Fixative 4F:1G for EM samples

[Mike Safron]

Trumps Fixative 
Ten ml of 40% formaldehyde and 2 ml of 50% glutaraldehyde were added to 200-mOsm buffer. 
This solution could be conveniently prepared for routine use as follows: 
1.16 g of NaH[2]PO[4] is added to 88 ml water and dissolved. 
Add 0.27 g NaOH followed by the formaldehyde and the glutaraldehyde. 
Ref. McDowell, E and B. Trump. 1976. Histological fixatives for diagnostic light and electron microscopy. Arch. Pathol. Lab. Med. 100:405-414. 
 
Michael A Safron A.S., HT (ASCP)CM
WIL Research Laboratories LLC
Manager, Histology
419-289-8700 ext 3040