[Histonet] Metanil Yellow and Methyl Green

[Igor Deyneko]

Dear Histonetters!
I have a question regarding a dye called Metanil Yellow, can anyone suggest
a good vendor for this dye (I know Sigma and Polyscientific sell it) and
what are your impressions? Second question, I have asked long time regarding
Methyl Green. I narrowed it down to DAKO ready to use, Polyscientific and
also Vector. I did the usual stain for 5 mins and then quick rinse with 95%
ethanol, and then 10 dips in series of 2 95%ethanols, 2X 100% and then 2
mins in 2 changes of Xylene and still have inconsistencies. Can someone
suggest a good protocol? Thank you very much.
Igor Deyneko
Infinity Pharmaceuticals
In-Vivo Pharmacology
Cambridge, MA 02139