[Histonet] PLS Help with IHC protocol

[Rene J Buesa]

Yes, it will.
René J.

--- On Tue, 9/30/08, Kimberly Tuttle <ktuttle <@t> umm.edu> wrote:

From: Kimberly Tuttle <ktuttle <@t> umm.edu>
Subject: RE: [Histonet] PLS Help with IHC protocol
To: "Patsy Ruegg" <pruegg <@t> ihctech.net>, histonet <@t> pathology.swmed.edu
Date: Tuesday, September 30, 2008, 1:06 PM

Its 1.1mg/ml

Another question: they are asking that I add glycerol to the antibody to
prevent freezing. Wont that change my concentration?

Kimberly C. Tuttle  HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland 
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax 


>>> "Patsy Ruegg" <pruegg <@t> ihctech.net> 9/30/2008 11:52
am >>>
Kim,
We need to know what the concentration of the antibody is before you make it
10ug/ml

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net 
web site www.ihctech.net 
 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kimberly
Tuttle
Sent: Tuesday, September 30, 2008 9:25 AM
To: histonet <@t> pathology.swmed.edu 
Subject: [Histonet] PLS Help with IHC protocol

Step 10: Add antibody at 10ug/ml in PBS + 0.1%BSA + 1:10 volume CAS block

Can someone break this down for me in simple instructions? 

Kimberly C. Tuttle  HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland 
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax 


 

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