[Histonet] Re: Which one first - antigen retrieval or peroxidase block

[Rene J Buesa]

As far as I remember enzymes are proteins and are they not supposed to cross-link with formalin also?
René J.

--- On Mon, 9/29/08, Greg Dobbin <gvdobbin <@t> ihis.org> wrote:

From: Greg Dobbin <gvdobbin <@t> ihis.org>
Subject: Re: [Histonet] Re: Which one first - antigen retrieval or peroxidase block
To: AGrobe2555 <@t> aol.com, histonet <@t> lists.utsouthwestern.edu
Date: Monday, September 29, 2008, 10:12 PM

Peroxidase is an enzyme. It doesn't miraculously appear as a result to
exposure to heat.  You show me one one slide where this has occurred and
I will humbly remain silent in this forum for a whole month! Ok, maybe
just a week...  :-)
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"And in the end it's not the years in your life
that count. It's the life in your years." 
- Abraham Lincoln

>>> <AGrobe2555 <@t> aol.com> 09/29/08 5:59 PM >>>
 
I'm with Rene on this one.  Block after retrieval.
 
For those of you who do the block before retrieval, how do you  control
for 
any activity that might be exposed during the retrieval  step?  Couldn't
it 
lead to false positives in your slides? 
 
Albert C.  Grobe, PhD
Tissue Engineering Lab
International Heart Institute of Montana  Foundation




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