[Histonet] which one first - antigen retrieval or endo peroxidase block?

Rene J Buesa rjbuesa <@t> yahoo.com
Thu Sep 25 14:56:36 CDT 2008


Antigen retrieval always comes first.
René J.

--- On Thu, 9/25/08, Monfils, Paul <PMonfils <@t> Lifespan.org> wrote:

From: Monfils, Paul <PMonfils <@t> Lifespan.org>
Subject: [Histonet] which one first - antigen retrieval or endo peroxidase block?
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, September 25, 2008, 2:26 PM

When doing immunoperoxidase staining on a tissue that requires antigen
retrieval, which do you do first - the antigen retrieval or the endogenous
peroxidase blocking?  Or does it matter?
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