AW: [Histonet] Gi Biopsies and the Tissue Tek VIP

[Gudrun Lang]

Thanks for the info. We use the sponges and I doubt, that a short protocol
is compatible with the fully filled cassettes.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprüngliche Nachricht-----
Von: Laurie Colbert [mailto:laurie.colbert <@t> huntingtonhospital.com] 
Gesendet: Donnerstag, 25. September 2008 16:17
An: gu.lang <@t> gmx.at
Betreff: RE: [Histonet] Gi Biopsies and the Tissue Tek VIP

We do not use sponges - most are wrapped in filter paper.  We process
anywhere from 40-100 biopsy cassettes daily.

Laurie

-----Original Message-----
From: Gudrun Lang [mailto:gu.lang <@t> gmx.at] 
Sent: Wednesday, September 24, 2008 11:45 PM
To: Laurie Colbert
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] Gi Biopsies and the Tissue Tek VIP

Laurie,
do you use the biopsy-sponges with this protocol? And how many cassettes do
you process at one time?

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Laurie
Colbert
Gesendet: Mittwoch, 24. September 2008 22:52
An: Amy Johnson
Cc: histonet <@t> lists.utsouthwestern.edu
Betreff: RE: [Histonet] Gi Biopsies and the Tissue Tek VIP

We have all stations set at 10 minutes, except for the last xylene and
the last two paraffins - which are 15 minutes each.
We actually have the two formalins at one hour and one and a half hours,
in case someone mistakenly puts the big tissue on the bx processor - at
least they will be well-fixed before we have to re-process them.  We
have plenty of time to have the formalin at longer times, but if your
specimens are already fixed, you should be able to go with the 10
minutes.

Laurie Colbert
Huntington Hospital
(626) 397-8620

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy
Johnson
Sent: Wednesday, September 24, 2008 11:39 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Gi Biopsies and the Tissue Tek VIP

Can anyone out there help me figure out what is the shortest amount of
time you can process GI biopsies with a Tissue Tek VIP?  

Thanks 

Amy Johnson 

Associates in Pathology

 

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