[Histonet] RE: Biocare IHC instrument
Goodwin, Diana
GoodwinD <@t> pahosp.com
Tue Sep 23 14:19:00 CDT 2008
I was very impressed with the Biocare instrument. It is called the Intellipath. Open system (my favorite feature), continuous feed up to 10 slides per load, able to run 5 protocols simultaneously for a total of 50 slides running simultaneously- can't remember how many protocols it can store. Definitely worth a look.
Diana Goodwin
Anatomic Pathology
Pennsylvania Hospital
Philadelphia, PA.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, September 23, 2008 1:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 58, Issue 27
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."
Today's Topics:
1. Re: Microtome micron calibration and validation
(Randall Carpenter)
2. Re: Humidity levels in the lab (Robert Richmond)
3. RE: Oversized microscope supply source (IN USA) (Jerry Helisek)
4. Histology schools/training in San Antonio, TX (Jackie M O'Connor)
5. Microtome (histobeach <@t> aol.com)
6. Seattle-Tacoma craigslist: Manager/Senior Manager, Histology
and Laboratory Resources (Bothell, WA) (Owen, Michael P)
7. Re: Cassette Holders (Joseph Saby)
8. BioCare (Jon Google)
9. Re: BioCare (Roxanne Soto)
10. Cryosectioning dorsal mouse skin (Orlowski Christian)
11. detection of brain infarcts (Sohail Ejaz)
12. Re: Blind Review Policy (mprice26 <@t> juno.com)
13. RE: Cassette Holders (Ford Royer)
14. Effect of 5%Aeti acid on Nuclei (Trajkovic, Dusko)
15. Re: Effect of 5%Aeti acid on Nuclei (Rene J Buesa)
16. Re: Re: Blind Review Policy (mprice26 <@t> juno.com)
17. Re: Cassette Holders (HACKERLAB <@t> aol.com)
18. RE: Ventana Retic Stain (Joyce Cline)
----------------------------------------------------------------------
Message: 1
Date: Mon, 22 Sep 2008 10:53:19 -0700 (GMT-07:00)
From: Randall Carpenter <tchistology <@t> earthlink.net>
Subject: [Histonet] Re: Microtome micron calibration and validation
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6919864.1222106000120.JavaMail.root <@t> elwamui-little.atl.sa.earthlink.net>
Content-Type: text/plain; charset=UTF-8
I just did an IQ, OQ, PQ on my process. The OQ on the microtome involved using a dial micrometer. Set the thickness at 5 microns. Tare or zero the dial micrometer on a point on your sample holder. Pull the micrometer point back, turn the wheel X number of times and take your new reading. It should add up to X times 5 microns +/- your tolerances. The rest of the "validation" process depends on it being installed properly and that it actually cuts sections suitable for pathologic interpretation. As we all know, just because the dial on the microtome says 5, doesn't mean the section is 5 microns. The point of the Operation Qualification is only to demonstrate that the machine works like it's supposed to. The dial micrometer needs to be calibrated/traceable. I got a guy in town to do it for $20. Of course everything needs to be documented and QA should sign off on it. Hope it helps.
Randy Carpenter
Twin Cities Histology
Hi,
I want to know ur comments and thoughts on microtome micron calibration and validation.Is there any method for doing it,please explain
Thanks and Regards
B.Radhika
Junior Scientist
Preclinical safety evaluation
Dr Reddy's Discovery Research
------------------------------
Message: 2
Date: Mon, 22 Sep 2008 13:53:29 -0400
From: "Robert Richmond" <RSRICHMOND <@t> aol.com>
Subject: [Histonet] Re: Humidity levels in the lab
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<abea52a60809221053q5d70816cw55d2cd254bba760e <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
>>In the summertime [the humidity in our laboratory] is at around
>>60-70%, which seems to work well. In the winter time it is less than 0
>>and I run a humidifier full out to try to achieve 20%.<<
If your relative humidity is less than zero, you're probably putting too much thiotimoline in your water bath!
(If you're too young for this literary allusion, see http://en.wikipedia.org/wiki/Thiotimoline
) and make the acquaintance of the late great Isaac Asimov!
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 3
Date: Mon, 22 Sep 2008 14:04:56 -0400
From: "Jerry Helisek" <Jerry <@t> ralambusa.com>
Subject: [Histonet] RE: Oversized microscope supply source (IN USA)
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: borsay.dodi <@t> epamail.epa.gov
Message-ID:
<3855F92002259948A66A8CA2D16E3A4F0B5CA8 <@t> server.ralambusa.com>
Content-Type: text/plain; charset="us-ascii"
We also supply many larger sizes, see www.ralamb.com
Thanks,
------------------------------------
Raymond A. Lamb, Inc
Jerry Helisek
VP North America
jerry <@t> ralambusa.com
5409 Lumley Road, Unit 102
Durham, North Carolina 27703
tel: 919.957.1964
fax: 919.957.1972
mobile: 919.264.7964
Skype ID:jerryhelisek
------------------------------------
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, September 22, 2008 1:09 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 58, Issue 26
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific than
"Re: Contents of Histonet digest..."
Today's Topics:
1. Oversized microscope slide source (borsay.dodi <@t> epamail.epa.gov)
2. Microtome micron calibration and validation
(radhikab <@t> drreddys.com)
3. Beta-Gal antibody (Dustin Hambright)
4. CE opportunity (Jennifer MacDonald)
5. RE: Humidity levels in the lab (Burton, Lynn)
----------------------------------------------------------------------
Message: 1
Date: Tue, 16 Sep 2008 14:06:57 -0400
From: borsay.dodi <@t> epamail.epa.gov
Subject: [Histonet] Oversized microscope slide source
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFBAF8E3EE.5D3D7259-ON852574C6.006376A4-852574C6.00638136 <@t> epamail.epa.g
ov>
Content-Type: text/plain; charset=US-ASCII
Here's a website for a company based in California, US.
http://www.tedpella.com/histo_html/slides-large.htm
The UK distributor is:
Agar Scientific Ltd
66a Cambridge Road
Stansted, Essex CM24 8DA
United Kingdom
Tel.: 44-1279-813519
sales <@t> agarscientific.com
http://www.agarscientific.com
Dodi Borsay Horowitz, Biologist
US Environmental Protection Agency
Atlantic Ecology Division
27 Tarzwell Drive
Narragansett, Rhode Island 02882
401/782-3042
borsay.dodi <@t> epamail.epa.gov
------------------------------
Message: 2
Date: Fri, 19 Sep 2008 11:19:08 +0530
From: radhikab <@t> drreddys.com
Subject: [Histonet] Microtome micron calibration and validation
To: Histonet <@t> lists.utsouthwestern.edu, clarkda <@t> ohsu.edu
Message-ID:
<OF7366F7BA.6D1998DB-ON652574C9.001F9BEA-652574C9.001FF6DE <@t> drreddys.com>
Content-Type: text/plain; charset="us-ascii"
Hi,
I want to know ur comments and thoughts on microtome micron calibration and validation.Is there any method for doing it,please explain
Thanks and Regards
B.Radhika
Junior Scientist
Preclinical safety evaluation
Dr Reddy's Discovery Research
Disclaimer
This message contains legally privileged and/or confidential information. If you are not the intended recipient(s), or employee or agent responsible for delivery of this message to the intended recipient(s), you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited.
If you have received this message in error, please immediately notify the sender and delete this e-mail message from your computer.
WARNING: Computer viruses can be transmitted via email. The recipient should check this email and any attachments for the presence of viruses.
The company accepts no liability for any damage caused by any virus transmitted by this email.
------------------------------
Message: 3
Date: Mon, 22 Sep 2008 11:44:22 -0400
From: Dustin Hambright <hambrightd <@t> mail.nih.gov>
Subject: [Histonet] Beta-Gal antibody
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C4FD3596.104%hambrightd <@t> mail.nih.gov>
Content-Type: text/plain; charset="US-ASCII"
Hello,
Does anyone know a reliable source for a Beta-Gal (lac-z) antibody for mouse frozen immunohistochemistry? Do you know the company and dilution?
Thank you in advance,
Dustin
------------------------------
Message: 4
Date: Mon, 22 Sep 2008 09:18:43 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: [Histonet] CE opportunity
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFFDF8AD43.E440794F-ON882574CC.00597C78-882574CC.0059C10A <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"
Continuing Education Opportunity (3 CE units) Diagnostic BioSystems is sponsoring a workshop in cooperation with the California Society for Histotechnology.
The workshop will be held at Mt. San Antonio College.
Date: Saturday, October 18
Time: 9:30 AM to 1:00 PM
Title: IHC: Basics and Beyond
Maximum attendance: 60
For more information or to RSVP, please contact Jennifer MacDonald at jmacdonald <@t> mtsac.edu
------------------------------
Message: 5
Date: Mon, 22 Sep 2008 11:38:32 -0500
From: "Burton, Lynn" <Lynn.Burton <@t> Illinois.gov>
Subject: RE: [Histonet] Humidity levels in the lab
To: "Thomas Jasper" <tjasper <@t> copc.net>, "Randolph-Habecker, Julie"
<jhabecke <@t> fhcrc.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<B34ED7468185B44AB6CECF566B1960D001D87768 <@t> IL084EX110.illinois.gov>
Content-Type: text/plain; charset="iso-8859-1"
In the summertime ours is at around 60-70%, which seems to work well. In the winter time it is less than 0 and I run a humidifier full out to try to achieve 20%. It is definitely easier in the summer.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Thomas Jasper
Sent: Fri 9/19/2008 5:01 PM
To: Randolph-Habecker, Julie
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Humidity levels in the lab
Julie,
I can't offer you any references, but here's something to consider. A cool mist humidifier in your lab. We are in Bend, OR., on the dry side of the Cascades. Running the humidifier reduces static and makes sectioning a bit easier. No hard science with this, just a little more atmospheric moisture.
By the way, the humidifier I bought is a big blue and white penguin.
The mist streams out the beak. Just looking at it makes you smile. I bought it at Target for ~ $40.00 - it works for us.
Have a good weekend.
Tom J.
Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services Bend, Oregon 97701
541/693-2677
tjasper <@t> copc.net
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Randolph-Habecker, Julie
Sent: Thursday, September 18, 2008 3:37 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Humidity levels in the lab
Folks,
We just moved to a new lab space in a new building. We are currently running at 45% or lower humidity. I am working with our facilities on raising the level but I am looking for some references for what level we should aim for in regard to paraffin cutting. Does anyone have some ideas or places to look for information?
Thanks!!
Julie
Julie Randolph-Habecker, Ph.D.
Staff Scientist - Director
Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024
206-667-6119
jhabecke <@t> fhcrc.org
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 58, Issue 26
****************************************
------------------------------
Message: 4
Date: Mon, 22 Sep 2008 14:54:20 -0500
From: Jackie M O'Connor <Jackie.O'Connor <@t> abbott.com>
Subject: [Histonet] Histology schools/training in San Antonio, TX
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OFA2093C12.90AE1AD5-ON862574CC.006D33DB-862574CC.006D5E1D <@t> abbott.com>
Content-Type: text/plain; charset="US-ASCII"
Can someone advise me of the schools available in the San Antonio area, and what the prerequisites are?
Thanks,
Jackie
------------------------------
Message: 5
Date: Mon, 22 Sep 2008 16:28:07 -0400
From: histobeach <@t> aol.com
Subject: [Histonet] Microtome
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CAEB08DB6DE055-EF4-16E5 <@t> webmail-nc12.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Hello,
We will be purchasing another microtome and I wanted to get feedback from the list.? If you wouldn't mind giving me your thoughts on the microtome you like the best.? We are not getting an automated one, not in our budget, but a simple, ever-lasting one.? We have the Leica 2135 and 2125 and sometimes the blade holder gives us problems, but I'm sure they all do.? Actually, we do have a Microm 330 and that one hasn't given us any problems at all.
I just would like to know the one that gets a lot of positive feedback.
Thanks so much in advance,
Stacey, HT
Biomed?Group
------------------------------
Message: 6
Date: Mon, 22 Sep 2008 16:30:48 -0400
From: "Owen, Michael P" <michael.owen <@t> fda.hhs.gov>
Subject: [Histonet] Seattle-Tacoma craigslist: Manager/Senior Manager,
Histology and Laboratory Resources (Bothell, WA)
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<449E51C6DA0AD840B44F57C7A6EB07BF04220657 <@t> FMD3VS022.fda.gov>
Content-Type: text/plain; charset="us-ascii"
seattle-tacoma craigslist / jobs / biotech/sci
http://seattle.craigslist.org
http://seattle.craigslist.org/see/sci/850615554.html
Manager/Senior Manager, Histology and Laboratory Resources (Bothell, WA)
------------------------------------------------------------------------
--------
Reply to: see below
Date: 2008-09-22, 9:23AM PDT
Would you like to work for a premier provider of innovative solutions in
drug discovery and development? MDS Pharma Services has an exciting
opportunity as a Manager/Senior Manager, Histology and Laboratory
Resources at our Bothell, WA location.
In this full-time position, you will be responsible for the management
of our histology and bioassay laboratory and staff in support of
preclinical efficacy pharmacology studies. The job is expected to be
split approximately 70% managerial functions and 30% histology or
bioassay laboratory bench work. Technical experience in the preparation
of paraffin, plastic and/or frozen tissue specimens is required. As the
laboratory manager, you will develop and administer budgets, performance
requirements, and schedules. You will also monitor and control labor and
capital expenditures and will interpret results of laboratory
activities. You will provide operational oversight of the laboratory and
technical guidance with regard to feasibility and timing to project
teams. Other staff functions include interviewing, hiring, and training
employees as well as addressing staff complaints and oversight of the
development of new methods or assays.
A BS/BA degree in a scientific field and 8+ years of experience is
desired along with 2+ years of supervisory/management experience.
Equivalent education and experience is also acceptable. Experience in
histology and /or, histomorphometry is required. Experience with ELISA,
RIA or clinical pathology laboratory procedures are highly desirable.
Experience in working skeletal or CNS diseases is a plus. Familiarity
with GLP and CLIA requirements are desirable as well.
AA/EEO
Please apply on-line at www.mdsps.com.
Location: Bothell, WA
Compensation: Great pay and benefits!
Principals only. Recruiters, please don't contact this job poster.
Please, no phone calls about this job!
Please do not contact job poster about other services, products or
commercial interests.
PostingID: 850615554
Michael P. Owen, Regulatory Microbiologist
U.S. FDA Pacific Regional Lab Northwest
22201 23rd Drive SE Bothell, WA 98021-4421
Phone: 425-483-4865 E-Mail: michael.owen <@t> fda.hhs.gov
------------------------------
Message: 7
Date: Mon, 22 Sep 2008 17:00:37 -0700 (PDT)
From: Joseph Saby <saby_joseph_a <@t> yahoo.com>
Subject: Re: [Histonet] Cassette Holders
To: histoinfo <@t> comcast.net, Histonet <@t> lists.utsouthwestern.edu
Message-ID: <857916.89293.qm <@t> web33804.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Jennifer-
Be of good cheer!
I saw them at the NSH convention.
Now if I could only remember where...
Joe
----- Original Message ----
From: "histoinfo <@t> comcast.net" <histoinfo <@t> comcast.net>
To: Histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, September 18, 2008 3:47:34 PM
Subject: [Histonet] Cassette Holders
Greetings Netters,
For those of you that remember the old metal cassette holders. It seems that they are no longer available (no surprise), and I cant find anything like them on the market. They were sold by tissue-tek I think. They are about 4 inches square and one inch tall. They have 5 rows that hold 3 cassettes each for a total of 15 cassettes. We are looking for more of these, if anyone has some they are no longer using please contact me.
Many Thanks,
Jennifer Sauners HT (ASCP)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Mon, 22 Sep 2008 17:11:33 -0700 (PDT)
From: Jon Google <ht.ascp <@t> yahoo.com>
Subject: [Histonet] BioCare
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <681179.96425.qm <@t> web59916.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Did anyone see the new biocare IHC stainer at NSH? could you give your impressions? We are looking at the Bond, Leica Bond, but we have just heard about the biocare system.
Thanks in advance,
------------------------------
Message: 9
Date: Mon, 22 Sep 2008 20:33:18 -0400
From: Roxanne Soto <godsgalnow <@t> aol.com>
Subject: Re: [Histonet] BioCare
To: "ht.ascp <@t> yahoo.com" <ht.ascp <@t> yahoo.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <9E679682-16C0-43F1-B524-588DD966F603 <@t> aol.com>
Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes
This happens to be my favorite of all the newer instruments. If it
does everything proposed-wow, what a time saver it would be. To be
able to load it without the other program being finished. DAKO tried
an instrument like this a couple of years ago but it bombed. I would
set yourself up for a demo. Once they are released they are going to
go quick- we are getting 4 of them throughout our system.
Sent from my iPhone
On Sep 22, 2008, at 8:11 PM, Jon Google <ht.ascp <@t> yahoo.com> wrote:
> Did anyone see the new biocare IHC stainer at NSH? could you give
> your impressions? We are looking at the Bond, Leica Bond, but we
> have just heard about the biocare system.
>
> Thanks in advance,
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Tue, 23 Sep 2008 14:02:15 +1000
From: "Orlowski Christian" <Christian.Orlowski <@t> petermac.org>
Subject: [Histonet] Cryosectioning dorsal mouse skin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C7845B83B0803D42A6EC7E29C99312848166F7 <@t> PMC-EMAIL.petermac.org.au>
Content-Type: text/plain; charset="us-ascii"
Greetings all,
I'm wondering whether there are any recommendations for how to
effectively cryosection dorsal mouse skin for immunohistochemistry. I'm
having difficulty with much of the mouse skin slipping off the slide
during the fixation and washing steps, which seems to result in loss of
the epidermis in particular, that part of the tissue that I happen to be
interested in. I apply the dorsal mouse skin cryosections onto
SuperFrost Plus (Menzel-Glaser) slides and air dry them for 2 hours. I
use acetone for fixation, 10 minutes, and use PBS for washing. Any
suggestions?
This email (including any attachments or links) may contain
confidential and/or legally privileged information and is
intended only to be read or used by the addressee. If you
are not the intended addressee, any use, distribution,
disclosure or copying of this email is strictly
prohibited.
Confidentiality and legal privilege attached to this email
(including any attachments) are not waived or lost by
reason of its mistaken delivery to you.
If you have received this email in error, please delete it
and notify us immediately by telephone or email. Peter
MacCallum Cancer Centre provides no guarantee that this
transmission is free of virus or that it has not been
intercepted or altered and will not be liable for any delay
in its receipt.
------------------------------
Message: 11
Date: Tue, 23 Sep 2008 03:37:21 -0700 (PDT)
From: Sohail Ejaz <sohail_e <@t> yahoo.com>
Subject: [Histonet] detection of brain infarcts
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <551193.17390.qm <@t> web39503.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
HI Everybody
I am trying to find some stain that can detect brain infarct.
Plreviously i was using TTC, but now we have ban on that.
So, looking forward for a best alternative.
Thanks
Sohail
------------------------------
Message: 12
Date: Tue, 23 Sep 2008 12:07:08 GMT
From: "mprice26 <@t> juno.com" <mprice26 <@t> juno.com>
Subject: [Histonet] Re: Blind Review Policy
To: sohail_e <@t> yahoo.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20080923.070708.18021.0 <@t> webmail06.dca.untd.com>
Content-Type: text/plain; charset=windows-1252
Does anyone have a Blind Case review Policy they could share with me? This is where when there is only one Pathologist and he does not have another pathologist to do peer review,In a one Pathologist lab.
Is anyone else doing this?
Thank you.
Marsha Price
____________________________________________________________
Click to consolidate your debt in minutes, stop late or over-limit fees, pay less.
http://thirdpartyoffers.juno.com/TGL2141/fc/Ioyw6i3m2bkZgujH4vUMxAF4GxmsePRHuCIW6Y6ywMqxb9PWv5ADBn/
------------------------------
Message: 13
Date: Tue, 23 Sep 2008 08:37:15 -0500
From: "Ford Royer" <froyer <@t> bitstream.net>
Subject: RE: [Histonet] Cassette Holders
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BD43ED6E40E64AE383B45ADA4F1DA6A1 <@t> Ford>
Content-Type: text/plain; charset="iso-8859-1"
I am not sure if this is what you are looking for but it is the one that I
am familiar with that is still being manufactured.
http://www.innovativelabacrylics.com/products/pages/cassette.html
Ford M. Royer, MT(ASCP)
Histology Product Manager
Minnesota Medical, Inc.
7177 Madison Ave. W.
Golden Valley, MN 55427-3601
CELL: 612-839-1046
Phone: 763-542-8725
Fax: 763-546-4830
Web: http://www.minnesotamedical.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joseph Saby
Sent: Monday, September 22, 2008 7:01 PM
To: histoinfo <@t> comcast.net; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Cassette Holders
Jennifer-
Be of good cheer!
I saw them at the NSH convention.
Now if I could only remember where...
Joe
----- Original Message ----
From: "histoinfo <@t> comcast.net" <histoinfo <@t> comcast.net>
To: Histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, September 18, 2008 3:47:34 PM
Subject: [Histonet] Cassette Holders
Greetings Netters,
For those of you that remember the old metal cassette holders. It seems that
they are no longer available (no surprise), and I cant find anything like
them on the market. They were sold by tissue-tek I think. They are about 4
inches square and one inch tall. They have 5 rows that hold 3 cassettes each
for a total of 15 cassettes. We are looking for more of these, if anyone has
some they are no longer using please contact me.
Many Thanks,
Jennifer Sauners HT (ASCP)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Tue, 23 Sep 2008 06:58:05 -0700
From: "Trajkovic, Dusko" <dusko.trajkovic <@t> pfizer.com>
Subject: [Histonet] Effect of 5%Aeti acid on Nuclei
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3AD0BD3142459B4E9B12CBEAFF2B89B2080F9C1C <@t> lajamrexm01.amer.pfizer.com>
Content-Type: text/plain; charset="us-ascii"
Good day to Everyone and for those that attended the NSH, WELCOME BACK.
I would hope that the parties were good and I am truly sorry that I had
to miss all of them.
I have a friend who asked me to post this for him. Any answer or
suggestion would be appreciated.
Thank you
Dusko Trajkovic
I'm asking because in a paper I'm reviewing a paper where someone is
using
a concentration of 45-60% Acetic acid to fix isolated nuclei, then
fixing again with Carnoy's, and then doing FISH (fluorescence in situ
hyb) on them.
I'm trying to figure out the effects on chromatin...does it cause the
nuclei and/or chromatin to swell?
I'd appreciate if you could ask around.
Thanks,
Yun
------------------------------
Message: 15
Date: Tue, 23 Sep 2008 07:11:22 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Effect of 5%Aeti acid on Nuclei
To: Histonet <@t> lists.utsouthwestern.edu, "Trajkovic, Dusko"
<dusko.trajkovic <@t> pfizer.com>
Message-ID: <509501.27454.qm <@t> web65703.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Yes, the immediate effect of acetic is nuclear swelling.
René J.
--- On Tue, 9/23/08, Trajkovic, Dusko <dusko.trajkovic <@t> pfizer.com> wrote:
From: Trajkovic, Dusko <dusko.trajkovic <@t> pfizer.com>
Subject: [Histonet] Effect of 5%Aeti acid on Nuclei
To: Histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, September 23, 2008, 9:58 AM
Good day to Everyone and for those that attended the NSH, WELCOME BACK.
I would hope that the parties were good and I am truly sorry that I had
to miss all of them.
I have a friend who asked me to post this for him. Any answer or
suggestion would be appreciated.
Thank you
Dusko Trajkovic
I'm asking because in a paper I'm reviewing a paper where someone is
using
a concentration of 45-60% Acetic acid to fix isolated nuclei, then
fixing again with Carnoy's, and then doing FISH (fluorescence in situ
hyb) on them.
I'm trying to figure out the effects on chromatin...does it cause the
nuclei and/or chromatin to swell?
I'd appreciate if you could ask around.
Thanks,
Yun
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 16
Date: Tue, 23 Sep 2008 14:47:51 GMT
From: "mprice26 <@t> juno.com" <mprice26 <@t> juno.com>
Subject: Re: [Histonet] Re: Blind Review Policy
To: mprice26 <@t> juno.com
Cc: histonet <@t> lists.utsouthwestern.edu, sohail_e <@t> yahoo.com
Message-ID: <20080923.094751.6345.0 <@t> webmail10.dca.untd.com>
Content-Type: text/plain; charset=windows-1252
Does anyone have a policy for a Blind Case Review for a one pathologist private lab?
Thank you.
Marsha Price
____________________________________________________________
Internet Security Software - Click here.
http://thirdpartyoffers.juno.com/TGL2141/fc/Ioyw6i3mEWrnsLu04AAPUy8TIFwZG9ifrjPeX3laAPLUa845TgE3Sp/
------------------------------
Message: 17
Date: Tue, 23 Sep 2008 11:53:17 EDT
From: HACKERLAB <@t> aol.com
Subject: Re: [Histonet] Cassette Holders
To: histoinfo <@t> comcast.net
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <c6c.3436f07a.360a6aed <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Hello Jennifer;
In response to your search for cassette holders, please check out this link:
_http://www.hackerinstruments.com/labstorage.htm_
(http://www.hackerinstruments.com/labstorage.htm)
They were on display at the NSH meeting in Pittsburgh.
Best regards,
Elfi Hacker
In a message dated 9/18/2008 3:48:03 P.M. Eastern Daylight Time,
histoinfo <@t> comcast.net writes:
Greetings Netters,
For those of you that remember the old metal cassette holders. It seems that
they are no longer available (no surprise), and I cant find anything like
them on the market. They were sold by tissue-tek I think. They are about 4
inches square and one inch tall. They have 5 rows that hold 3 cassettes each for
a total of 15 cassettes. We are looking for more of these, if anyone has some
they are no longer using please contact me.
Many Thanks,
Jennifer Sauners HT (ASCP)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
**************Looking for simple solutions to your real-life financial
challenges? Check out WalletPop for the latest news and information, tips and
calculators. (http://www.walletpop.com/?NCID=emlcntuswall00000001)
------------------------------
Message: 18
Date: Tue, 23 Sep 2008 12:48:14 -0400
From: "Joyce Cline" <jcline <@t> wchsys.org>
Subject: RE: [Histonet] Ventana Retic Stain
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BBA106E703A74B9D90EF2440669A8EC0 <@t> wchsys.org>
Content-Type: text/plain; charset="us-ascii"
We have to let our retic slides dry for 1 hour at 72C. We use superfrost
plus slides otherwise we have variable staining or no staining.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Thursday, September 18, 2008 3:18 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ventana Retic Stain
Is anyone out there having problems with their Retic stain on the
Ventana Nexus Special Stainer? Please contact me offline.
Laurie Colbert
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
***** CONFIDENTIALITY NOTICE *****
This message contains confidential information and is intended only for
the individual named. If you are not the named addressee you should not
disseminate, distribute or copy this e-mail. Please notify the sender
immediately by e-mail if you have received this e-mail by mistake and
delete this e-mail from your system.
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 58, Issue 27
****************************************
The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message.
More information about the Histonet
mailing list