[Histonet] Glycerol jelly

[Jo Dee Fish]

Hi Pumla,
Our recipe includes 1ml or liquified Phenol per 150ml of Jelly.
Good luck,
Jo Dee 


~~Jo Dee Fish~~
Senior Research Technologist
The J. David Gladstone Institutes
Co-manager Histology and Microscopy Core
 
Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail: jfish <@t> gladstone.ucsf.edu
 
Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pumla
Pamla-Gutter
Sent: Tuesday, September 23, 2008 11:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Glycerol jelly

I've recently started using home-made glycerol jelly as my mounting
medium---composition gelatin powder, water, and glycerol. I keep it in the
fridge at 4 degrees and melt it before use. It's great for dark field
images, but I'm worried about bacterial growth. I haven't had any problems
yet, but I was wondering if someone else out there uses this and what they
do to prevent the growth of mold, etc... and how long do you keep it before
you toss it?
Thanks,
Pumla Pamla-Gutter
(614)292-4046

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