[Histonet] Cryosectioning dorsal mouse skin

[Orlowski Christian]

Greetings all,

 

I'm wondering whether there are any recommendations for how to
effectively cryosection dorsal mouse skin for immunohistochemistry. I'm
having difficulty with much of the mouse skin slipping off the slide
during the fixation and washing steps, which seems to result in loss of
the epidermis in particular, that part of the tissue that I happen to be
interested in. I apply the dorsal mouse skin cryosections onto
SuperFrost Plus (Menzel-Glaser) slides and air dry them for 2 hours. I
use acetone for fixation, 10 minutes, and use PBS for washing. Any
suggestions?



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