[Histonet] Question on staining

[Herrick, James L.]

Hi all,

I am trying to stain for osteoid and bone in pig tibia (need to quantify osteoid and bone volumes). Does anyone have experience with these types of stains in 40-60 µm thick, MMA embedded, sections? I have tried a Masson's trichrome and Toluidine blue, but have been unable to get well defined stain differentiation with this thick of sections. I would really appreciate any help I can get. Thanks again.

Jim