[Histonet] question about Her2 neu

[Rene J Buesa]

The ASCO/CAP recommendation is 6 to 8 hours fixation in NBF and even this fixation period does not assure an effective crosslinking. The fact that they are biopsies is irrelevant because the NBF fixation time is the same for specimens from 160µm to 4 mm thick (the crosslinking time is independent of tissue thickness).
You cannot compare your results to others unless you know exactly how your methodology compares. M<methodology and the IHC procedure itself (reagent and their dilutions) can influence the difference between your results and the others.
My advise is to run your procedure according to the ASCO/CAP standards and do not worry about other results. Working properly will back the quality of your results.
René J.

--- On Mon, 9/15/08, Green, Kathy <kgreen <@t> hsh.org> wrote:

From: Green, Kathy <kgreen <@t> hsh.org>
Subject: [Histonet] question about Her2 neu
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, September 15, 2008, 9:05 AM

First, is there a time frame involved for fixation for formalin issue
when running Her2neu?  If it is FDA approved is it necessary to monitor
it (time frames).  We are having a problem on our trials that the ours
are running higher (ours would be a 1+ vs. 0) than the company we
previously sent them to.  Could it be due to the fact that they don't
have an FDA approved HER 2 neu and maybe not from the same company (we
use Ventana antibodies/equipment) that we use?  Thanks for any help or
pointers in this area.

 

Please respond to my personal email any responses.

 

Kathy Green, HT

Manager Histology Laboratory

Holy Spirit Hospital

503 N. 21st Street

Camp Hill, PA  17011

kgreen <@t> hsh.org

(717) 763-2930

Blackberry:  (717) 370-1726