[Histonet] antigen retrieval for 5 year pap smear slides

[hao zhang]

Hi, everyone

I want to do IHC from a 5 year old pap smear slides for pan-keratin stain.

I tried many methods of antigen retrieval protocols (10 mM citric buffer, ph
6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at 95C for 40
minutes. I also used Triton x 100 (0.2%) pretreatment 10 minutes and then
SDS (0.5% or 0.25%) treatment for 10 minutes. But all method did not work.
Any replies will be very appreciated.


-- 
Hao ZHANG, Ph.D

Scientist