[Histonet] RE: Formaldehyde and scrubs

Tue Sep 9 13:53:17 CDT 2008

Lets see.....   naked histology tech walking the halls, eating in the cafeteria....  Yummm

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Madary, Joseph
Sent: Tuesday, September 09, 2008 2:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formaldehyde and scrubs

 What I like is when people who wear scrubs walk the hallways and even eat in the cafeteria with blood, tissue, and smelling of chemicals on their scrubs.  I really like that a lot.

Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Laboratory Mgr
One Medimmune Way, Lab 2438-Area 4
Gaithersburg, MD 20878

ph 301.398.4745/6360
fx  301.398.9745

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, September 09, 2008 1:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 58, Issue 10

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Today's Topics:

   1. VEGFR1 and 2 (Hale, Sarah)
   2. Re: VEGFR1 and 2 (Nancy  Lemke)
   3. Using Ventana with new antibody lots (Sharon.Davis-Devine)
   4. RE: Air purifiers (Tony Henwood)
   5. RE: Air purifiers (Tony Henwood)
   6. Varistain Gemini Auto Stainer (Leblanc, Barbara Ann)
   7. RE: Varistain Gemini Auto Stainer (Blazek, Linda)
   8. Re: Using Ventana with new antibody lots (Rene J Buesa)
   9. Re: Varistain Gemini Auto Stainer (Rene J Buesa)
  10. RE: Using Ventana with new antibody lots (Gwen Deville)
  11. Formaldehyde and scrubs (Laurie Colbert)
  12. Re: Formaldehyde and scrubs (Rene J Buesa)
  13. Re: Formaldehyde and scrubs (Jackie M O'Connor)
  14. Re: Formaldehyde and scrubs (Jennifer MacDonald)
  15. RE: Formaldehyde and scrubs (McMahon, Loralee A)

----------------------------------------------------------------------

Message: 1
Date: Mon, 8 Sep 2008 13:36:38 -0400
From: "Hale, Sarah" <Sarah.Hale <@t> uvm.edu>
Subject: [Histonet] VEGFR1 and 2
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <36830148D2AC3E48A318C1436C89462B088BED78 <@t> MED05.med.uvm>
Content-Type: text/plain;       charset="US-ASCII"

Hi!

Does anyone have any experience with IHC for VEGFR1 and 2 in rat arteries?  Our lab has had trouble getting good staining especially with paraffin sections.

Thanks!
-Sarah

__________________________________________________
Sarah Hale, Ph.D.
Postdoctoral Associate
Dept. of OB/Gyn and Reproductive Sciences
C215 Given Bldg.
University of Vermont
Burlington, VT  05405
ph: 802.656.1207
email: sarah.hale <@t> uvm.edu

------------------------------

Message: 2
Date: Mon, 08 Sep 2008 13:53:16 -0400
From: "Nancy  Lemke" <nsnwl <@t> neuro.hfh.edu>
Subject: Re: [Histonet] VEGFR1 and 2
To: "Hale, Sarah" <Sarah.Hale <@t> uvm.edu>,
        histonet <@t> lists.utsouthwestern.edu
Message-ID: <d9b08c2c0bcf8b3e056c4994f7ec5a23 <@t> neuro.hfh.edu>
Content-Type: text/plain; charset=us-ascii

Hi Sarah,
I can heartily recommend Santa Cruz sc-6251.  FFPE: retrieve in citrate buffer, pH 6.0- boil for 10 minutes, cool for 20.  Block, then incubate in sc-6251 at 1:1500 for 30 minutes at room temp.  I use Biocare 4+ detection as given on data sheet and Biocare Betazoid DAB for 4 minutes and get beautiful, clean results.  I use this in rat sections.

Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit
-----Original message-----
From: "Hale, Sarah" Sarah.Hale <@t> uvm.edu
Date: Mon, 08 Sep 2008 13:37:43 -0400
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] VEGFR1 and 2

> Hi!
>
> Does anyone have any experience with IHC for VEGFR1 and 2 in rat
> arteries?  Our lab has had trouble getting good staining especially
> with paraffin sections.
>
> Thanks!
> -Sarah
>
> __________________________________________________
> Sarah Hale, Ph.D.
> Postdoctoral Associate
> Dept. of OB/Gyn and Reproductive Sciences
> C215 Given Bldg.
> University of Vermont
> Burlington, VT  05405
> ph: 802.656.1207
> email: sarah.hale <@t> uvm.edu
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

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Message: 3
Date: Mon, 8 Sep 2008 16:15:05 -0500
From: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>
Subject: [Histonet] Using Ventana with new antibody lots
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <44780C571F28624DBB446DE55C4D733A021E0A94 <@t> EXCHANGEBE1.carle.com>
Content-Type: text/plain;       charset="us-ascii"

Ok, Histonetters I have another question for you.

According the CAP checklist question:

ANP.22750  Phase II

Has the laboratory documented evaluation of new antibody lots and new antibodies, prior to use in patient diagnosis?

NOTE:  For newly introduced antibodies, staining conditions should be evaluated in cases expected to be positive and negative for the antigen of interest. Ideally, a series of sufficient size should be run to give the laboratory an idea of the sensitivity and specificity of the test.

How are you folks handling this question when using Ventana?

Thanks a bunch.

Sharon Davis-Devine, CT (ASCP)

Cytology Supervisor

Carle Clinic

602 West University

Urbana, Illinois 61801

Phone:  217-383-3572

Email:  sharon.davis-devine <@t> carle.com

------------------------------

Message: 4
Date: Tue, 9 Sep 2008 08:49:07 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Air purifiers
To: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>,
        <histonet <@t> lists.utsouthwestern.edu>
Cc: Rex Johnson <rexcjohnson <@t> msn.com>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555202FB003D <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"

Are you sure that it not the hydrogen peroxide that you may be allergic to. The concentrated (30%) form will bleach your fingers if you are not wearing gloves (or if there is a pinhole in them!) making them quite itchy.

I am not aware of any cases of DAB allergy, though you may be the first or, most probably I need to research this more.

Is there anyone else who might have a DAB allergy?

We probably need to have our OH&S committee look into it.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira
Sent: Tuesday, 9 September 2008 1:39 AM
To: histonet <@t> lists.utsouthwestern.edu
Cc: mickie25 <@t> netzero.net; Rex Johnson
Subject: [Histonet] Air purifiers

Hi histonetters,

Is there any Air purifiers for DAB?

I guess I am allergic to that reagent; I can test it after using.

I am OK to Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone because I use them in the hood, but DAB is a step in autostainer.

Regards,

Naira

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
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------------------------------

Message: 5
Date: Tue, 9 Sep 2008 09:43:52 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Air purifiers
To: <rjbuesa <@t> yahoo.com>, <histonet <@t> lists.utsouthwestern.edu>,
        "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Cc: Rex Johnson <rexcjohnson <@t> msn.com>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555202FB003E <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="iso-8859-1"

Ozone may indeed be of some concern. Some research notes:

"High levels of atmospheric ozone in Florence air correlated with DNA damage, and to the prevalence of inflammatory pathologies of the upper respiratory tract, although the ozone concentrations were below the Italian recommended attention level. Furthermore, higher levels of DNA damage were correlated with a dysfunction in the ability to maintain a normal epithelial cell structure. These data suggest an association between ozone air levels and damage in the upper respiratory tract. It remains unclear whether ozone itself or other associated pollutants are responsible for the observed alterations." (Environ. Mol. Mutagen. 42:127-135, 2003)

"Beagle dogs exposed for 8 hours a day to 3 ppm O3 for 18 months showed cytologic changes that indicate metabolic alterations. The endoplasmic reticulum of the type 2 alveolar epithelial cells in the proximal alveoli were frequently dilated and contained a moderately electron-dense substance having a periodicity of approximately 754 A. This condition was accompanied by a substantial reduction in the lamellar membranes in the characteristic lamellar bodies of the type 2 cells, suggesting a sequestering of protein in the endoplasmic reticulum. In addition, endothelial cells contained paracrystalline arrays of cytoplasmic membranes not observed in control animals" (Am J Pathol. 1973 December; 73(3): 711-726)

"The interaction of ozone with some molecules results in an increased production of free radicals. The objective of this study was to identify whether acute ozone exposure to 1-1.5 ppm for 4 h, produced cytological and ultrastructural modifications in the olfactory bulb cells. The results showed that in rats exposed to ozone there was a significant loss of dendritic spines on primary and secondary dendrites of granule cells, whereas the control rats did not present such changes. Besides these exposed cells showed vacuolation of neuronal cytoplasm, swelling of Golgi apparatus and mitochondrion, dilation cisterns of the rough endoplasmic reticulum. These findings suggest that oxidative stress produced by ozone induces alterations in the granule layer of the olfactory bulb, which may be related to functional modifications" (Neuroscience Letters Volume 274, Issue 1, 15 October 1999, Pages 1-4)

"Inhalation of the pulmonary irritant ozone is associated with an accumulation of macrophages in the lung. These cells, along with type II epithelial cells, are activated to release increased quantities of hydrogen peroxide and nitric oxide, two reactive mediators that have been implicated in tissue injury" (Am J Respir Cell Mol Biol. 1996 Jun ;14 (6):516-25).

"ozone-induced high-protein alveolar edema is pathogenetically linked to pulmonary hyperemia, deficiency of surfactant tubular myelin, and associated lung dysfunctions" (Toxicol Appl Pharmacol Vol. 117 Issue 1 Pg. 37-45 Nov 1992).

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, 9 September 2008 2:47 AM
To: histonet <@t> lists.utsouthwestern.edu; Margaryan, Naira
Cc: Rex Johnson; mickie25 <@t> netzero.net
Subject: Re: [Histonet] Air purifiers

If you can taste DAB after working with it (either weighing of diluting it) it means that you need to wear an AIR MASK, the air purifier will not be enough. Remember that DAB is cancerigenous. René J.

--- On Mon, 9/8/08, Margaryan, Naira <NMargaryan <@t> childrensmemorial.org> wrote:

From: Margaryan, Naira <NMargaryan <@t> childrensmemorial.org>
Subject: [Histonet] Air purifiers
To: histonet <@t> lists.utsouthwestern.edu
Cc: "mickie25 <@t> netzero.net" <'mickie25 <@t> netzero.net'>, "Rex Johnson" <rexcjohnson <@t> msn.com>
Date: Monday, September 8, 2008, 11:39 AM

Hi histonetters,

Is there any Air purifiers for DAB?

I guess I am allergic to that reagent; I can test it after using.

I am OK to Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone because I use them in the hood, but DAB is a step in autostainer.

Regards,

Naira

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************

------------------------------

Message: 6
Date: Tue, 9 Sep 2008 06:03:09 -0500
From: "Leblanc, Barbara Ann" <blebl2 <@t> lsuhsc.edu>
Subject: [Histonet] Varistain Gemini Auto Stainer
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
        <E6D5527ABEF9BD43BF9149D64CBD5FBB04F2E57C <@t> umc-exch03.master.lsuhsc.edu>

Content-Type: text/plain;       charset="us-ascii"

Hi everyone:

We have a Varistain Gemini ES autostainer.  We are having variability in the stain quality of the slides.  Some slides are stained properly while others are stained lighter.  This is sometimes on the same slide (top section stained good; bottom section not good); sometimes some slides in the rack will stain okay and others in the same rack will not.  Does anyone have this stainer and has experienced this problem?

Thank you,
Barbara

------------------------------

Message: 7
Date: Tue, 9 Sep 2008 07:21:15 -0400
From: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
Subject: [Histonet] RE: Varistain Gemini Auto Stainer
To: "'Leblanc, Barbara Ann'" <blebl2 <@t> lsuhsc.edu>,
        "histonet <@t> pathology.swmed.edu" <histonet <@t> pathology.swmed.edu>
Message-ID:
        <5A2BD13465E061429D6455C8D6B40E39119A05C3 <@t> IBMB7Exchange.digestivespecialists.com>

Content-Type: text/plain; charset="us-ascii"

Barbara,
I don't have that stainer but have had that problem from time to time.  If you are using recycled alcohol in your last alcohol station or if your alcohol is not really at 100% that may be the problem.
Linda

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lblazek <@t> digestivespecialists.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Leblanc, Barbara Ann
Sent: Tuesday, September 09, 2008 7:03 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Varistain Gemini Auto Stainer

Hi everyone:

We have a Varistain Gemini ES autostainer.  We are having variability in the stain quality of the slides.  Some slides are stained properly while others are stained lighter.  This is sometimes on the same slide (top section stained good; bottom section not good); sometimes some slides in the rack will stain okay and others in the same rack will not.  Does anyone have this stainer and has experienced this problem?

Thank you,
Barbara
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 8
Date: Tue, 9 Sep 2008 05:25:22 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Using Ventana with new antibody lots
To: histonet <@t> lists.utsouthwestern.edu,  "Sharon.Davis-Devine"
        <Sharon.Davis-Devine <@t> carle.com>
Message-ID: <397080.62015.qm <@t> web65709.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

This applies to ALL new lots or antibodies, not just for Ventana.
1- when I received a new lot, I tested it WITHOUT  a patient section (just with a + control) before using it with patients. The results were documented in the Abs log.
2- the other question refers to the introduction of a new Ab, something you had not used before. In that case you have to run it with a series of potentially + controls, at different dilution rates to determine the working concentration and the suitability for the purpose it is intended for. It is like a validation test and should be documented also. The pathologist has to sign the validation and concentration tests.
René J.

--- On Mon, 9/8/08, Sharon.Davis-Devine <Sharon.Davis-Devine <@t> carle.com> wrote:

From: Sharon.Davis-Devine <Sharon.Davis-Devine <@t> carle.com>
Subject: [Histonet] Using Ventana with new antibody lots
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, September 8, 2008, 5:15 PM

Ok, Histonetters I have another question for you.

According the CAP checklist question:

ANP.22750  Phase II

Has the laboratory documented evaluation of new antibody lots and new antibodies, prior to use in patient diagnosis?

NOTE:  For newly introduced antibodies, staining conditions should be evaluated in cases expected to be positive and negative for the antigen of interest. Ideally, a series of sufficient size should be run to give the laboratory an idea of the sensitivity and specificity of the test.

How are you folks handling this question when using Ventana?

Thanks a bunch.

Sharon Davis-Devine, CT (ASCP)

Cytology Supervisor

Carle Clinic

602 West University

Urbana, Illinois 61801

Phone:  217-383-3572

Email:  sharon.davis-devine <@t> carle.com

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 9
Date: Tue, 9 Sep 2008 05:43:14 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Varistain Gemini Auto Stainer
To: histonet <@t> pathology.swmed.edu, "Leblanc, Barbara Ann"
        <blebl2 <@t> lsuhsc.edu>
Message-ID: <613411.20328.qm <@t> web65707.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Confronted with an instrument problem that I could not solve by myself, the best option always is to contact the instrument vendor. They usually are able to help. I advise you to do the same.
René J.

--- On Tue, 9/9/08, Leblanc, Barbara Ann <blebl2 <@t> lsuhsc.edu> wrote:

From: Leblanc, Barbara Ann <blebl2 <@t> lsuhsc.edu>
Subject: [Histonet] Varistain Gemini Auto Stainer
To: histonet <@t> pathology.swmed.edu
Date: Tuesday, September 9, 2008, 7:03 AM

Hi everyone:

We have a Varistain Gemini ES autostainer.  We are having variability in the stain quality of the slides.  Some slides are stained properly while others are stained lighter.  This is sometimes on the same slide (top section stained good; bottom section not good); sometimes some slides in the rack will stain okay and others in the same rack will not.  Does anyone have this stainer and has experienced this problem?

Thank you,
Barbara
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 10
Date: Tue, 9 Sep 2008 08:46:19 -0500
From: "Gwen Deville" <gdeville <@t> deltapathology.com>
Subject: RE: [Histonet] Using Ventana with new antibody lots
To: <rjbuesa <@t> yahoo.com>, <histonet <@t> lists.utsouthwestern.edu>,
        "'Sharon.Davis-Devine'" <Sharon.Davis-Devine <@t> carle.com>
Message-ID: <003901c91282$708d59d0$51a80d70$@com>
Content-Type: text/plain;       charset="iso-8859-1"

I recently asked CAP for specific details of how antibody validation should be handled since there are many different opinions out there, from lab to lab, tech to tech, and CAP and CLIA.  Especially with CLIA enforcing more regs on the anatomic pathology lab.  In the past, we in AP did not feel like many of these regs fit in our department, most seemed to be more geared to the clinical laboratory.  After experiencing a CLIA visit, I can tell you we have changed our thinking.  Dr. Patrick Fitzgibbons responded to my question.  There is a book that CAP publishes that every laboratory should have called, "QUALITY MANAGEMENT IN ANATOMIC PATHOLOGY".  Chapter 8 deals specifically with IHC and Antibody validation.  It details to what extent they should be tested prior to patient testing.  Who would know better what is expected in this area?

Gwen Deville, Histology Supv.
Delta Pathology, Mid-Louisiana
Box 30113, 211 Fourth Street
Alexandria, LA 71301

Work: (318)473-3943 / (318) 473-3180
Pager: (318) 427-5444
Email: gdeville <@t> deltapathology.com

NOTICE:  This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient.  If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissenmination, distribution, forwarding, printing, or copying of the email or any attached files is strictly prohibited.  If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, September 09, 2008 7:25 AM
To: histonet <@t> lists.utsouthwestern.edu; Sharon.Davis-Devine
Subject: Re: [Histonet] Using Ventana with new antibody lots

This applies to ALL new lots or antibodies, not just for Ventana.
1- when I received a new lot, I tested it WITHOUT  a patient section (just with a + control) before using it with patients. The results were documented in the Abs log.
2- the other question refers to the introduction of a new Ab, something you had not used before. In that case you have to run it with a series of potentially + controls, at different dilution rates to determine the working concentration and the suitability for the purpose it is intended for. It is like a validation test and should be documented also. The pathologist has to sign the validation and concentration tests.
René J.

--- On Mon, 9/8/08, Sharon.Davis-Devine <Sharon.Davis-Devine <@t> carle.com>
wrote:

From: Sharon.Davis-Devine <Sharon.Davis-Devine <@t> carle.com>
Subject: [Histonet] Using Ventana with new antibody lots
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, September 8, 2008, 5:15 PM

Ok, Histonetters I have another question for you.

According the CAP checklist question:

ANP.22750  Phase II

Has the laboratory documented evaluation of new antibody lots and new antibodies, prior to use in patient diagnosis?

NOTE:  For newly introduced antibodies, staining conditions should be evaluated in cases expected to be positive and negative for the antigen of interest. Ideally, a series of sufficient size should be run to give the laboratory an idea of the sensitivity and specificity of the test.

How are you folks handling this question when using Ventana?

Thanks a bunch.

Sharon Davis-Devine, CT (ASCP)

Cytology Supervisor

Carle Clinic

602 West University

Urbana, Illinois 61801

Phone:  217-383-3572

Email:  sharon.davis-devine <@t> carle.com

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 11
Date: Tue, 9 Sep 2008 07:07:20 -0700
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Subject: [Histonet] Formaldehyde and scrubs
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <57BE698966D5C54EAE8612E8941D768303ACD1BE <@t> EXCHANGE3.huntingtonhospital.com>

Content-Type: text/plain;       charset="us-ascii"