[Histonet] amyloid control for congo red

Jackie M O'Connor Jackie.O'Connor <@t> abbott.com
Fri Sep 5 13:43:11 CDT 2008


Amyloid degrades on cut sections.    Slides have to be cut fresh for 
optimal results.

Jackie O'



"Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
09/05/2008 12:52 PM

To
"Nancy Schmitt" <nancy_schmitt <@t> pa-ucl.com>, 
<histonet <@t> lists.utsouthwestern.edu>
cc

Subject
RE: [Histonet] amyloid control for congo red






We try not to cut amyloid controls too far in advance - we have had
trouble with old slides and poor staining in the past.

Laurie

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nancy
Schmitt
Sent: Friday, September 05, 2008 9:33 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] amyloid control for congo red

Happy Friday Histonetters,

Can amyloid control slides be cut ahead?  Should the block be dipped in
paraffin after each use?  Having a bit of trouble with this stain  -
have a
new block coming - but want to move forward doing this properly. 

Thanks for your help
Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA


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