[Histonet] anti-collagenase staining paraffine tissue

[D.E.Hilling <@t> lumc.nl]

Does anybody have any experience with anti-collagenase staining on
paraffine sections? 

In frozen sections we get a sensible staining pattern (the way we
expected it to be), but we have problems transferring this to paraffine
sections of the same tissue (human pancreasses), were the staining seems
to be less intense and atypical (despite changing concentrations,
duration). 

I reckon, it might be a cross-linking problem (due to the formaline
fixation en paraffine embedding??) ..... does anybody know if (and how)
I can get rid of these or maybe have other suggestions? 

 

Thanks!

 

Denise Hilling

 

 

Denise Hilling MD

PhD student

Department of Surgery

Leiden University Medical Center

The Netherlands