[Histonet] BUGZ

Wed Sep 3 13:27:50 CDT 2008

Hi Amos,
You can decalcify with hydrochloric acid with a chelating agent like EDTA.
0.5M EDTA pH 8.0 (GIBCO) 30ml
ddH2O 70ml
6N HCL 1.2ml
pH 6.4-6.8
1. Wash fixed tissue with PBS  20min X3.
2. Wash tissue with ddH2O 20min X3.
3. Change tissue to EDTA solution with agitation at RT for 2-3 days 
depend on tissue size.
4. Change to fresh EDTA solution if need further decalcification.
5. Rinse with ddH2O 20min X3.
6. Wash with ddH2O 20min X3.
7. Dehydration and embedding
I did not see much in the insect world but there is some info in the 
invertebrate world.
Lots of exoskeleton and chitin there.
Hope this helps.
Lori

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>    1. Michelle Steinkrauss is out of the office.
>       (michelle.steinkrauss <@t> novartis.com)
>    2. RE: Documenting control tissue source? (Cheri Miller)
>    3. Job opening (Rathborne, Toni)
>    4. NSH Conference (Suzanne Bruce)
>    5. Differentiating a neutrophil from a de-granulated	eosinophil
>       ....antibody or stain? (jstaruk)
>    6. Re: Differentiating a neutrophil from a de-granulated
>       eosinophil ....antibody or stain? (Mark Tarango)
>    7. BUGZ (Amos Brooks)
>    8. Histology Position Available -  Baltimore Maryland
>       (LAURA KORCZYNSKI)
>    9. IHC buffer expiration date (Jan Shivers)
>   10. Hello (Lori Charette)
>   11. Re: Hello (Pat Flannery)
>   12. What to do when there is no liquid nitrogen :p (Heath, Nancy L.)
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> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 2 Sep 2008 13:36:24 -0400
> From: michelle.steinkrauss <@t> novartis.com
> Subject: [Histonet] Michelle Steinkrauss is out of the office.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<OFB36FB263.F23D5E8B-ON852574B8.0060B79C-852574B8.0060B79C <@t> ah.novartis.com>
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>
> I will be out of the office starting  09/02/2008 and will not return until
> 09/03/2008.
>
> If you require immediate assistance for study-related matters, please
> contact Michelle Broome at x 47477.  For clinical pathology assistance,
> please contact Rhonda Osborne at x 47099.
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 2 Sep 2008 14:05:48 -0500
> From: "Cheri Miller" <cmiller <@t> physlab.com>
> Subject: RE: [Histonet] Documenting control tissue source?
> To: "'Tom McNemar'" <TMcNemar <@t> lmhealth.org>,
> 	<histonet <@t> pathology.swmed.edu>
> Message-ID: <00a101c90d2e$e95fdbc0$3d02a8c0 <@t> plab.local>
> Content-Type: text/plain;	charset="us-ascii"
>
> I agree with you. We just had our inspection in July and it wasn't an issue.
>
>
> Cheryl Miller HT (ASCP)
> Histology Supervisor
> Physicians Laboratory,P.C.
> Omaha, Ne. 
> 402 738 5052
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom McNemar
> Sent: Friday, August 15, 2008 6:12 AM
> To: histonet <@t> pathology.swmed.edu
> Subject: [Histonet] Documenting control tissue source?
>
> We are finishing up our JCAHO inspection today.  The inspector asked about
> our control tissues and where we documented the source.  We get nearly all
> of our controls from patient samples and she said that most histo labs mark
> their control blocks with the source case number.  They then keep records of
> the control source used when staining new cases.  I can see no logical
> reason to keep this info.  You use a control appropriate for the element
> being stained.  Why does it matter where the control came from?  Am I wrong?
> Do you do this?  
>
> Thanks.
>
> Tom McNemar, HT(ASCP)
> Histology Co-ordinator
> Licking Memorial Health Systems
> (740) 348-4163
> (740) 348-4166
> tmcnemar <@t> lmhealth.org
> www.LMHealth.org
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> ------------------------------
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> Message: 3
> Date: Tue, 2 Sep 2008 15:26:52 -0400
> From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
> Subject: [Histonet] Job opening
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
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> Somerset Medical Center, located in central NJ, has a daytime opening for a FT Histotech. Embedding, microtomy, knowledge of special stains and IHC required. Apply online at http://somersetmedicalcenter.com/, or send resume to:
>
> trathborne <@t> somerset-healthcare.com
>
> Toni Rathborne
> Pathology Supervisor
> Somerset Medical Center
> 110 Rehill Ave.
> Somerville,NJ 08876
> 908-595-2367
>  
>
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>
> ------------------------------
>
> Message: 4
> Date: Tue, 2 Sep 2008 16:51:40 -0400
> From: "Suzanne Bruce" <sbruce <@t> vetpathservicesinc.com>
> Subject: [Histonet] NSH Conference
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> 	<26DB1FDFBF9EE14AB3AACC873519A4A2515149 <@t> vpss1.VetPathServicesInc.local>
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>
> Hello, this message is mainly for the boneheads and plastics users (specifically Technovit 7200, GMA and others using the Exakt system).  I will be attending the NSH conference for the first time.  I don't know many people involved in plastics, so I was wondering who else would be attending that is familiar w/the plastics I mentioned above.  I'd certainly like to meet a few of you!  Also any friendly words of advice about getting the most out of the conference would be appreciated!
>  
> Thanks so much!
> ________________________________________
> Suzanne Bruce,  R.V.T.
> Histologist / Necropsy Coordinator
> Vet Path Services, Inc. (VPS)
> 6450 Castle Dr.
> Mason, OH 45040
> Lab:  (513) 469-0777 ext 19
> Fax:  (513) 469-2474
> Email:  sbruce <@t> vetpathservicesinc.com
> www.vetpathservicesinc.com <http://www.vetpathservicesinc.com/> 
> <https://69.61.197.115/exchweb/bin/redir.asp?URL=http://www.vetpathservicesinc.com/>  
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 2 Sep 2008 20:26:31 -0400
> From: "jstaruk" <jstaruk <@t> masshistology.com>
> Subject: [Histonet] Differentiating a neutrophil from a de-granulated
> 	eosinophil ....antibody or stain?
> To: "'histonet'" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <A66EE4BC17994DAD8787B366BC091A38 <@t> JimPC>
> Content-Type: text/plain;	charset="US-ASCII"
>
> Hi all,
>
> Does anyone know if the anti-eosinophil antibody will adhere to a
> degranulated eosinophil or does the antibody only stick to the eosinophil
> granules and not the cell itself?
>
> I'm performing a differential count in a certain fluid and I'm highly
> suspicious that many of the neutrophils are actually de-granulated
> eosinophils (a bit larger, thicker, less segmented nuclei, etc).  Is there
> any other method to differentiate a neutrophils from a degranulated
> eosinophil that I'm not aware of?
>
> Thank you!
>
> Jim
>
> _____________________
>      Jim Staruk
> Mass Histology Service
> www.masshistology.com
>
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 2 Sep 2008 17:32:14 -0700
> From: "Mark Tarango" <marktarango <@t> gmail.com>
> Subject: Re: [Histonet] Differentiating a neutrophil from a
> 	de-granulated	eosinophil ....antibody or stain?
> To: jstaruk <jstaruk <@t> masshistology.com>
> Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> 	<5b6eb13e0809021732j11d1c969nc41921d7f3628091 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Got a picture?
>
> On 9/2/08, jstaruk <jstaruk <@t> masshistology.com> wrote:
>   
>> Hi all,
>>
>> Does anyone know if the anti-eosinophil antibody will adhere to a
>> degranulated eosinophil or does the antibody only stick to the eosinophil
>> granules and not the cell itself?
>>
>> I'm performing a differential count in a certain fluid and I'm highly
>> suspicious that many of the neutrophils are actually de-granulated
>> eosinophils (a bit larger, thicker, less segmented nuclei, etc).  Is there
>> any other method to differentiate a neutrophils from a degranulated
>> eosinophil that I'm not aware of?
>>
>> Thank you!
>>
>> Jim
>>
>> _____________________
>>     Jim Staruk
>> Mass Histology Service
>> www.masshistology.com
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>     
>
>
>
> ------------------------------
>
> Message: 7
> Date: Wed, 3 Sep 2008 10:24:47 -0400
> From: "Amos Brooks" <amosbrooks <@t> gmail.com>
> Subject: [Histonet] BUGZ
> To: "histonet <@t> lists.utsouthwestern.edu"
> 	<histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> 	<582736990809030724p3a842a4fscedd9fc487f35c21 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi,
>    Here's an oddball question. I have a researcher that wants to bring me
> mosquitoes for paraffin sectioning. I immediatly had a chill when thinking
> of cutting the chitin. Has anyone worked with similar bugs, and if so how
> did you process them to make them cuttable? What fixative would be best?
>
> Thanks,
> Amos
>
>
> ------------------------------
>
> Message: 8
> Date: Wed, 03 Sep 2008 10:26:11 -0400
> From: "LAURA KORCZYNSKI" <Lkorczyn <@t> gbmc.org>
> Subject: [Histonet] Histology Position Available -  Baltimore Maryland
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <48BE6640.B3F2.00C2.0 <@t> gbmc.org>
> Content-Type: text/plain; charset=US-ASCII
>
>
>
> HISTOTECHNOLOGIST
> Full Time -Day Shift/1 Saturday Every 6 weeks
>
> Fully automated histology team is looking for an experienced individual that is ASCP registered or is eligible.  At least 2 years histology experience is desired.  Duties include embedding, cutting, H&E and Histochemical Staining of tissue specimens.  Free on-site parking.  Qualified candidates can apply on-line to GBMC.org and/or send resume to LKorczyn <@t> gbmc.org.
>
>
>
>
> Laura Korczynski
>
> Histology Supervisor 
> Greater Baltimore Medical Center
> Phone:  443-849-2831
> Fax:  443-849-3016 
>
> _______________________________________________________________________________________
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> ------------------------------
>
> Message: 9
> Date: Wed, 3 Sep 2008 09:49:20 -0500
> From: "Jan Shivers" <shive003 <@t> umn.edu>
> Subject: [Histonet] IHC buffer expiration date
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <3FEAA312E14E47989C362072225F5E04 <@t> auxs.umn.edu>
> Content-Type: text/plain;	charset="iso-8859-1"
>
> Hi folks,
>
> What is the general concensus on expiration dates of IHC buffers that one prepares from scratch and keeps at room temperature (TBS, Citrate, etc.)?  I've found one source that states 3 months as a storage time period.  Is this the general assumption or do you use a different timeline?
>
> Jan Shivers
> Histology/IHC/EM 
> University of Minnesota
>
>
> ------------------------------
>
> Message: 10
> Date: Wed, 03 Sep 2008 11:09:48 -0400
> From: Lori Charette <lac65 <@t> email.med.yale.edu>
> Subject: [Histonet] Hello
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <48BEA8BC.1070508 <@t> email.med.yale.edu>
> Content-Type: text/plain; format=flowed; charset=ISO-8859-1
>
> Hello
>
>   

-- 
Lori A. Charette, MSM, MT, HT (ASCP)
Manager, Research Histology / Tissue Microarray Facility
310 Cedar St.
LH-202
New Haven, CT 06510
phone (203)737-4198
fax (203) 737-1198