[Histonet] TUNEL on mouse bone marrow

[anh2006 <@t> med.cornell.edu]

I had the same experience and thought it was most likely due to the recombination that occurs in the BM during lymphocyte maturation - so the reagents are giving false positives. So instead I use cleaved caspase 3 IHC.


------Original Message------
From: FU,DONGTAO
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
To: Histonet <@t> lists.utsouthwestern.edu
Sent: Oct 23, 2008 9:15 AM
Subject: [Histonet] TUNEL on mouse bone marrow

Hi, all

  We met a problem with TUNEL staining on mouse BM(10% NBF fixed 
paraffin). The protocol we used works very well with other 
tissues, however, it gave us high background on bone marrow. Can 
anyone give us some suggestions on TUNEL staining using bone 
marrow?

  Many thanks,

Ann Dongtao Fu MD, Ph.D
Lab Manager
Dept. of Pathology
Lab phone: 352-273-7752
Lab FAX: 352-273-7755
Lab address: D11-50
PO Box: 100275
1600 SW Archer Road
University of Flodrida
Gainesville, FL 32610


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