[Histonet] Re: We are running a congo red stain and have siodium chloride cryatals precipa

[Jane M Flinn]

Thanks, the student indeed had a super saturated solution, Jane

"Life is short - make haste to be kind"

Dr. Jane Flinn
Director, Undergraduate Neuroscience Program
George Mason University, 3F5
4400 University Dr.
Fairfax, VA  22030
Phone: 703-993-4107
Fax: 703-993-1359


----- Original Message -----
From: renafail <@t> bellsouth.net
Date: Tuesday, October 14, 2008 1:54 pm
Subject: Re: [Histonet] Re: We are running a congo red stain and have siodium chloride cryatals precipa

> 
> Are you filtering the solutions before immersing the slides?  The 
> sodium chloride  is a  saturated solution.
> Rena Fail
> -------------- Original message from Jane M Flinn 
> <jflinn <@t> gmu.edu>: --------------
> 
> We are running a congo red stain and have sodium chloride crystals 
> precipataing 
> > onto the slide. Does anyone know why and how we can fix this? jane
> > 
> > "Life is short - make haste to be kind"
> > 
> > Dr. Jane Flinn
> > Director, Undergraduate Neuroscience Program
> > George Mason University, 3F5
> > 4400 University Dr.
> > Fairfax, VA  22030
> > Phone: 703-993-4107
> > Fax: 703-993-1359
> > 
> > 
> > ----- Original Message -----
> > From: kbowden 
> > Date: Wednesday, October 8, 2008 5:37 pm
> > Subject: Re: [Histonet] citrisolve?
> > 
> > > I have used Citrisolve for serveral years.  I don't think 
> anything 
> > > compares to Xylene, but I do think this comes very close.  I 
> > > change it 
> > > about once a week.  It pretty much deparaffinizes in the same 
> > > amount of 
> > > time.  Disposal is dependent on the regulations in your area.  
> > > When I 
> > > started using it our Environmental Health and Safety said pour 
> it 
> > > down 
> > > the drain, but as time goes by and with more regulations now I 
> > > have to 
> > > collect it and have it removed with hazardous waste.
> > > --
> > > Karen Bowden
> > > Staff Research Associate II
> > > University of CA, San Diego
> > > Department of Orthopedics
> > > 9500 Gilman Dr. 0630
> > > La Jolla, CA 92093-0630
> > > 858-534-4655 voice
> > > 858-534-5304 fax
> > > 
> > > 
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> > > 
> > > Patten, Nicole (NIH/NIAAA) [F] wrote:
> > > > Does anyone have any experience using Citrisolve as a Xylene 
> > > alternative> to deparaffinize tissue sections? Do I use it 
> just 
> > > like Xylene for the
> > > > same amount of time? Can it be reused or should I use new 
> Citrisolve> > > each time? How do I dispose of it? 
> > > >
> > > >  
> > > >
> > > > Any help at all would be much appreciated. Thanks!
> > > >
> > > >  
> > > >
> > > > Nicole J. Patten
> > > > Post-Baccalaureate Fellow/IRTA
> > > > National Institutes of Health
> > > >
> > > >  
> > > >
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>