Subject: [Histonet] SV40 HRP protocol HELP

[Troutman, Kenneth A]

This is our current protocol (on Dako Autostainer):
 

1.	
	Pretreat slides in Trilogy (Cell Marque) in pressure cooker for 20 minutes.
2.	
	Cool in hot soln for 10 minutes.
3.	
	Rinse in DI H2O.
4.	
	Peroxidase block for 5 min.
5.	
	Protein block for 5 min.
6.	
	SV-40 primary (Chemicon 1:10,000) for 30 min @ RT
7.	
	Dako Envision Dual link polymer for 30 min @ RT
8.	
	DAB 5 min.
9.	
	Counterstain, dehydrate, clear and coverslip.

Hope this helps.

Ashley Troutman BS, HT(ASCP)QIHC
Histopathology Laboratory
Department of Pathology
Vanderbilt University Medical Center
Nashville, TN
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