[Histonet] Antigen Retrieval

[Patten, Nicole (NIH/NIAAA) [F]]

Hi-

 

I am new to using IHC and I need some advice on Antigen Retrieval. I
usually do it in an autoclave for 15min at 121C for FFPE human brain
tissue (in Citrate or Tris Buffer), but by the end my tissue looks
pretty bad and parts have even fallen off. The tissue is fixed on
SuperFrost Plus Slides. 

 

Any suggestions would be really helpful. Thanks!

 

N. Patten
Post-Baccalaureate Fellow/IRTA
National Institutes of Health