[Histonet] Winterize formalin?

[Bryan Llewellyn]

When I was in Prince George, BC we used to tell our satellite labs to dilute 
the NBF 20% with ethanol .  This worked fine for us and below -20C.  At 
really cold temperatures it turned slushy rather than solid, but we rarely 
got ice crystal holes.

I used to live and work in Winnipeg.  I was the section head in histology at 
the HSC from 67-74.  How are things there, now?

Bryan Llewellyn


----- Original Message ----- 
From: "Sate Hamza" <dermpathsy <@t> gmail.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, October 02, 2008 7:58 AM
Subject: [Histonet] Winterize formalin?


> Dear Histonetters,
>
> A colleague from Boston posted this inquiry to our dermatopathology 
> mailing
> list: "Does anyone [have] the formula to winterize formalin? We were 
> adding
> 15 ml of alcohol to 1000 ml of 10% buffered formalin. It used to work but 
> is
> not working now at -20" ..
>
> Interestingly, I found out that we do not "winterize" formalin up here in
> Winnipeg, Canada (also jokingly referred to as "Winterpeg"). I am 
> wondering
> if anyone on the list "winterizes" formalin .. I found the following
> abstract of a Japanese paper by searching in Pubmed
>
> http://www.ncbi.nlm.nih.gov/pubmed/3692431
>
> Thank you ..
>
> Sate
>
> -- 
> Sate Hamza, MD, FRCPC
> Dermatopathologist
> Winnipeg, Canada
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