[Histonet] tissue swelling out of block

[Thomas Jasper]

Hi Gudrun,

Is your friend soaking blocks in ice water before cutting?  This is
common in animal histology and over soaking before  cutting will cause
swelling.  Different tissue types will swell more quickly than others,
but over soaking can eventually affect all tissue.

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, OR 97701
541/693-2677
tjasper <@t> copc.net

 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun
Lang
Sent: Wednesday, October 01, 2008 10:16 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] tissue swelling out of block

Hi all,

a friend recently got a job in a veterinary histo and has some troubles
in cutting. He tells about paraffinblocks, where the tissue is swelling
out while warming like a small hill.  I referred to the possibilty of
taking up humidity that causes swelling. 

Do you know other causes for this problem?

 

Thanks in advance

Gudrun Lang