[Histonet] gram stain controls

Walzer Susan Susan.Walzer <@t> HCAHealthcare.com
Wed Nov 26 02:16:58 CST 2008

We take two pieces of fresh tissue (umbilical cord works well) to micro and have them grow positive and negative on individual pieces. After a few days we fix the tissue and embed them together. Works great. PS we do the same when we need fungus controls.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, November 25, 2008 3:40 PM
To: histonet <@t> lists.utsouthwestern.edu; Fischer, R. B
Cc: Delcambre, Linda V
Subject: Re: [Histonet] gram stain controls

I always used actual tissues with gram +/- bacteria because it is not the same (time wise) to stain a histogel than a piece of tissue. Appendix is a good control.
René J.

--- On Tue, 11/25/08, Fischer, R. B <BFicher <@t> chomp.org> wrote:

From: Fischer, R. B <BFicher <@t> chomp.org>
Subject: [Histonet] gram stain controls
To: histonet <@t> lists.utsouthwestern.edu
Cc: "Delcambre, Linda V" <Linda.Delcambre <@t> chomp.org>
Date: Tuesday, November 25, 2008, 3:29 PM

     My pathologist has made a gram neg/pos control block for us to use.
He made this by taking separate cultures of each and suspended them in
histo gel. The control stains well. My question is: Since we are
performing gram stain on tissues exclusively, will this cultured control
be an accepted method as a control for tissues, or must we use tissue
with gram pos/neg organisms. Thanks for your reply.
R.Brian Fischer
Histology Lead Tech
Community Hospital of the Monterey Peninsula
PO Box HH Monterey Ca. 93942
Fax: 831-6583683
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