[Histonet] Warthin-Starry // "Best before" dates

[Lee & Peggy Wenk]

We make up the hydroquinone reducing solution just before use, but found for
the Steiner, since it makes up 60 mL, that if we use half and store the
other half in the refrig, we can use it the next day. After that, it doesn't
work very well.

As for silver solutions, if you are talking about a certain percent aqueous
silver nitrate (say 1% aq. Silver nitrate), we found aq. Silver nitrate is
good in the refrig for about 6 months, so we only make up enough to last 2-3
months, to be on the safe side. If you are talking about the silver made up
in a buffer solution, then I don't know how long it is good for.

If you don't do W-S very often, it just might be best if you make both of
these solutions up when the stain is requested. 

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of V. Neubert
Sent: Tuesday, November 25, 2008 2:49 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Warthin-Starry // "Best before" dates


Hello!  I use the Warthin-Starry technique to stain spirochaetes in FFPE
sections.  I don't know how long I can keep the AgNO_3 solutions and the
hydrochinone solution whitout getting them to "expire". Is there anyone who
already had to deal with that question?  Regards,  Valentin


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