[Histonet] Dissolve plastic

Joseph Saby saby_joseph_a <@t> yahoo.com
Mon Nov 24 17:10:45 CST 2008

Lee and Peggy-

We section these eyes all the time with the plastic in place.  

I would certainly suggest a gluaraldehyde fixative.  Straight NBF is a very poor fixative for the many tissues found in eyes, especially the retina.  If you contact me directly, we can talk off line.  Eyes have held a special fascination with me for many years.

Joe Saby

From: Lee & Peggy Wenk <lpwenk <@t> sbcglobal.net>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Monday, November 24, 2008 12:47:24 PM
Subject: [Histonet] Dissolve plastic

I Need Histonetters help.

We have an eye with a plastic part for the lens, which is a type of
telescope, I've been told.

According to the new pathologist, we need to dissolve out this plastic lens,
then he can gross the eye and we can process it into paraffin.

We've never had to dissolve plastic before. The eye is in 10% NBF right now.
I'm guess maybe something like chloroform? But what percent? For how long?
Before or after grossing?

Has anyone done this before, that can send a procedure to me? Thanks.


Peggy A. Wenk, HTL(ASCP)SLS
Program Director,  Schools of Histotechnology
Supervisor, Mortuary Services
Laboratory Safety Officer, Disaster Preparedness and Quality Assurance
Department of Anatomic Pathology, 100RO
William Beaumont Hospital
3601 W. 13 Mile Road
Royal Oak, MI 48073-6769
Work  248/898-9079
Fax      248/898-9054
E-mail  pwenk <@t> beaumont.edu
Web Page: http://www.beaumont.edu/alliedhealth 

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