[Histonet] Peggy - eye/plastic lens

[Paula Pierce]

I have processed many, many eyes with artificial lenses. The older lenses dissolved out in chloroform, however the newer plastics in use do not. I disect the colottes off either side of the iris and optic nerve and process the central section, IOL and all. 

When disecting, the haptics, which hold the IOL in place, will cut easily. If there is no fibrous tissue or lens capsule surrounding the IOL, it may dislodge during processing. But I have sectioned eyes with the IOL in place.

FYI, NBF will cause retinal detachment.

Paula 




________________________________
From: Vicki Kalscheur <kalschev <@t> svm.vetmed.wisc.edu>
To: Histonet Discussion <histonet <@t> pathology.swmed.edu>
Sent: Monday, November 24, 2008 12:35:17 PM
Subject: [Histonet] Peggy - eye/plastic lens

Hi Peggy:  It is possible that acetone ( swab on, or soak) will dissolve or loosen the lens without cellular damage. I have not done this and will be interested to see what other suggestions are made.  Vicki
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