[Histonet] Processing problems

Joe Nocito jnocito <@t> satx.rr.com
Thu Nov 20 05:32:18 CST 2008

it might be your processor.  Depending on the type of processor, some 
processors have a rotary valve that rotates from reagent bottle to reagent 
bottle. When the rotary valves fails, it starts placing a reagent in the 
reagents bottles indiscriminately. For instance, our processor started 
spitting paraffin in our alcohols.

----- Original Message ----- 
From: "Genest, Sharon SktnHR" <Sharon.Genest <@t> saskatoonhealthregion.ca>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, November 19, 2008 3:15 PM
Subject: [Histonet] Processing problems

We had a problem with our recycler and xylene contamination so we now
test each batch  by adding water to a sample of recycled alcohol. If it
turns cloudy we assumed that we had xylene contamination.
We have lately tested commercial ethanols (no turbidity)before placing
them on the processor and after we processing with them just once we
have turbidity after adding a water to all the ethanols 70-100%.
This is happening to all the alcohols on all of our processors. I am
thinking that it is not a xylene contamination but something else maybe
a leaching of plastic from the cassettes? Has this happened to anyone
else?  Can anyone give me any suggestions.
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