[Histonet] Re: Buffer.

[John Kiernan]

   PIPES-buffered  glutaraldehyde  has  been used for subsequent electron
   micr   artifacts    -      -&nbsp;when  used  for  perfu   <FONT  color=#0000ff>Schultz  RL   Membrane  alterations  in  cerebral cortex when   <EM>J.  Neurocytol.</EM>  <STRONG>1   461-469.</FONT>  The  authors  attributed  these  art   which  potentially  could  be mistaken for pathological changes,   the  low toxicity of PIPES. Apparently cacodylate or phosphate buff   er  prevented  the  metabolic changes that caused the artifacts during
   the     occur.  <   buffer  rather  tha   calcium   ions.   A   Ca   s   immobilization of phospholipids in cell   membranes.<BR>&nbsp;<BR>John   Kiernan<BR>Anatomy,
   UW   Messa   &lt;RSRICHMOND <@t> aol.com&   November  16,  2008 14:13<BR>Subjec   Buffer.<BR>To:                        histonet <@t> lists.utso   uthwestern.edu<BR><BR>&gt;  About the potential toxicity
   of   <BR>&gt;   &gt;&gt;The   the  Microscopy  Listserve   usually because someone is eith   exposure<BR>&gt;  or  has sa   the  stuff.  It was used in<BR>&g   phosphate  buffers  can  interact  with  some  <   the<BR>&gt;  fixative combinations and leave pre   Also,  I'd suspect,<BR>&gt; cacodylate is less   beasties  growing in stock solutions over<BR>&gt; time.   "modern"  zwitterionic  buffers are used a lot these <BR>&g   t;  days  -<BR>&gt; PIPES &amp; that crowd - if you want
   to         phosphate.&lt;&lt;   buffers  are  often referred to as Goo   or<BR>&gt;  Good  buffers,  since     Norman  Good  in the 1960's.<BR>&gt; S   http://en.wikipedia.org/wiki/Good's_buffers   <BR>&gt;  <BR>&gt;  I  don't  know why they aren't
   commo   fixatives.<BR>   Richmond<BR>&gt;    Samurai    Pathologi   Knoxville      TN<BR>&gt;      <BR>&gt;     ___   _______________________   5F   ____________________<BR>=2
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