[Histonet] therapeutic antibody detection in mouse xenografts?
Samuel_Perry <@t> DFCI.HARVARD.EDU
Wed Nov 19 13:22:57 CST 2008
I am wondering if anyone has experience showing therapeutic monoclonal antibody
binding in mouse xenografts (human tumor cells injected subcutaneously in SCID
mice and then dose treated with therapeutic antibody injected
intraperitoneally). For instance, can trastuzamab (Herceptin) binding be seen
in a breast tumor xenograft treated with Herceptin? I have tried using Dako's
goat-anti mouse dextran HRP conjugated secondary antibody developed with DAB. I
also tried using an Abcam goat-anti mouse antibody conjugated with biotin, then
adding Vectors elite ABC, then developing with DAB. Both times I used FFPE
tissue. In both techniques, results showed minimal to staining and showed no
difference between mice treated with antibody and control mice treated. I would
appreciate if anyone knew of published papers or have experience using IHC to
show therapeutic antibody bound to tumor cell in mouse xenografts.
Dana Farber Cancer Institute
The information transmitted in this electronic communication is intended only
for the person or entity to whom it is addressed and may contain confidential
and/or privileged material. Any review, retransmission, dissemination or other
use of or taking of any action in reliance upon this information by persons or
entities other than the intended recipient is prohibited. If you received this
information in error, please contact the Compliance HelpLine at 800-856-1983 and
properly dispose of this information.
More information about the Histonet