[Histonet] therapeutic antibody detection in mouse xenografts?

[Perry, Samuel]

Hi All,
I am wondering if anyone has experience showing therapeutic monoclonal antibody
binding in mouse xenografts (human tumor cells injected subcutaneously in SCID
mice and then dose treated with therapeutic antibody injected
intraperitoneally).  For instance, can trastuzamab (Herceptin) binding be seen
in a breast tumor xenograft treated with Herceptin?  I have tried using Dako's
goat-anti mouse dextran HRP conjugated secondary antibody developed with DAB.  I
also tried using an Abcam goat-anti mouse antibody conjugated with biotin, then
adding Vectors elite ABC, then developing with DAB.  Both times I used FFPE
tissue.  In both techniques, results showed minimal to staining and showed no
difference between mice treated with antibody and control mice treated.  I would
appreciate if anyone knew of published papers or have experience using IHC to
show therapeutic antibody bound to tumor cell in mouse xenografts. 
Thanks -Sam 

Research Technician
Dana Farber Cancer Institute
Boston MA.

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