[Histonet] RE: Uneven H&E Staining

[Karla Arrington]

Recently we have had an issue with uneven H&E staining.  Lately slides that have 
three sections on one slide, mostly GI biopsies, and the last section (bottom of 
slide) are paler than the upper two section.  All cut by the same person and the 
stains were great.  What could be the cause of this?  Also with the quality of 
formalin.  Can formalin be purchased as "bad" when it takes 6 days to fix placenta 
or breasts?  What is the cause? 
 
Karla Arrington, HT(ASCP)