[Histonet] C4d question (not CD4)

[Richard Cartun]

We have been using an "RUO"-labeled anti-C4d antibody on certain renal transplant cases for the immunohistochemical detection of humoral rejection for the past several years.  We have now been asked by our Transplant team to do this test routinely on all transplant kidney biopsies (which is another issue).

Is anyone using an "IVD"-labeled antibody for C4d detection by IHC?  Although we get excellent results with this antibody on formalin-fixed, paraffin-embedded tissue, I would prefer to be using an "IVD"-labeled antibody (if one exists).  Thanks.

Richard 

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax