[Histonet] Antibody protocol

Sebree Linda A. LSebree <@t> uwhealth.org
Tue Nov 18 11:56:39 CST 2008

This is our protocol for H S A from Biocare, clone OCHIE5:  mild CC1,
1:50 dilution for 24", AB block, Hem II/8".  If you don't get good
results with your CM antibody, try this one as we get beautiful, strong
but clean staining.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jesus
Sent: Tuesday, November 18, 2008 11:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Antibody protocol

Needing some help with the staining protocol for  HSA (Hepatocyte
Specific Antigen) clone OCH1E5, made by Cell marquee to be ran on the
Ventana LT/Benchmark platform.. Would anyone be willing to share
protocol information with us, as well as staining methodology.  Thank
you for the help.  


Jesus A Ellin  HT/PA  ASCP

Department of Pathology/Histology

Yuma Regional Medical Center

2400 South Ave A

Yuma, AZ  85364 - 7170

( Office:  (928) 336-1743

(    Fax:  (928) 336-7319

*    Email: jellin <@t> yumaregional.org 

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