[Histonet] HistoClear question

[Cheryl]

Hey Andi--

We had the same problem at a derm lab.  HistoClear has a companion product
for mounting media...it's the only one that works well (is it HistoMount or
NeoMount??).  Your supplier should have it listed alongside.  You'll be
AMAZED once you switch--a world of difference and no issues with water even
if your HistoClear isn't pristine.

Also--watch what alcohols you use right before the HistoClear.  Reagent or
something other than ethanol seems to work better.

Cheryl


Cheryl R. Kerry, HT(ASCP), BA
Full Staff Inc.
Staffing Healthcare Professionals - One GREAT fit at a time.
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea
Grantham
Sent: Tuesday, November 18, 2008 10:23 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HistoClear question

A student in my lab is doing some work in another lab here and having some
problems with coverslipping their H&E sections. They are using HistoClear 1X
as a clearing agent and Cytoseal mounting medium. From looking at the
coverslipped slides I'm guessing that these two products are not compatible.
The mounting medium is sort of clumping up under coverslips and has a hazy
appearance. Yes, I did also find a bit of water in their HistoClear as well.
Does anybody else have any experience with HistoClear 1X and/or does anybody
know what mounting medium is good to use with the product? I suggested that
they call the company who manufactures HistoClear but I don't know if they
have done that yet.

Thanks,

Andi Grantham
......................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html


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