[Histonet] Oil Red O despair - point of clarification

[Andrea Grantham]

I didn't mean that the propylene glycol and glycerin jelly were 
combined - just wanted to clear that up. The protocol uses both but 
for different things.

Andi Grantham



At 09:12 AM 11/18/2008, Andrea Grantham wrote:
>I get many projects here that require frozen liver sections and Oil 
>Red O staining. Some years ago I was having problems with the 
>staining and someone on histonet suggested the PolyScientific R & D 
>Oil Red O Stain Kit. I got the kit and have been using it ever 
>since. The stain works great - usually I have livers loaded with 
>lipid - and the slides come out clean. The kit uses proplyene glycol 
>and glycerin jelly to mount the coverslips. I do use one part of the 
>ORO protocol from Freida Carson's book - I fix the slides in 37-40% 
>(page 152, second edition).
>
>Andi Grantham
>
>
>
>
>At 04:49 PM 11/17/2008, Amos Brooks wrote:
>>Michele,
>>    You could try 0.5% Oil Red O in 60% triethylphosphate. That works really
>>well here. I've also used a formulation I found in Humanson, the same amount
>>of Oil Red O in 99% ethanol and it worked fairly well. Triethylphosphate is
>>much cleaner than the ethanol. (There was a precipitate on the slide.) I
>>can't avouch for isopropyl as I used ethanol, but it is good to know there's
>>an alternative in case we run out of triethylphosphate.
>>
>>Amos Brooks
>>
>>Message: 10
>>Date: Mon, 17 Nov 2008 11:56:45 -0600
>>From: "Michele Wich" <mwich <@t> 7thwavelabs.com>
>>Subject: [Histonet] Oil Red O despair
>>To: <histonet <@t> lists.utsouthwestern.edu>
>>Message-ID:
>>        <62A8156F8071C8439080D626DF8C33A602E554 <@t> wave-mail.7thwave.local>
>>Content-Type: text/plain;       charset="us-ascii"
>>
>>I'm wondering what are the pros (or cons) of using the propylene glycol
>>version of Oil Red O over the isopropanol method. Is one more suited to
>>a specific application?
>>
>>I'm trying to stain a frozen section of liver, which one would suspect
>>would be loaded with fat, and have thus far been unsuccessful using the
>>propylene glycol Oil Red O.
>>
>>Is there something obvious that I'm missing here? I'm new to
>>cryosectioning...perhaps I did something wrong in the cryostat. I fixed
>>my sections in NBF before staining.
>>
>>Please help. I'm feeling like a pathetic excuse for a histotech. Any
>>advice is greatly appreciated.
>>_______________________________________________
>>Histonet mailing list
>>Histonet <@t> lists.utsouthwestern.edu
>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>.....................................................................
>: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
>: Sr. Research Specialist       University of Arizona               :
>: (office:  AHSC 4212)          P.O. Box 245044                     :
>: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
>: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
>:...................................................................:
>           http://www.cba.arizona.edu/histology-lab.html
>
>
>_______________________________________________
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>

.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html