[Histonet] Biocare MM HRP or AP polymer kit on frozen sections

Gayle Callis gayle.callis <@t> bresnan.net
Mon Nov 17 15:52:10 CST 2008

Has anyone used this kit on frozen sections where formalin fixed paraffin embedded tissue is avoided entirely?   Biocare only has a data sheet for FFPE tissues, followed by the Decloaker or Decloaker/pepsin digestion protocols.  

For many of us using frozen sections exclusively with mouse on mouse IHC or IFA applications,  some guidelines would be appreciated for frozen sections that are not aldehyde fixed and these MM polymer kits.  Hopefully someone has played with this kit on fresh tissue frozen sections?   

Gayle M. Callis 
Bozeman MT 

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