[Histonet] Bone Marrow smears fixation

[Joyce Cline]

Toni,

They are fixed for 10 minutes in the fomalin fumes. We do not perform IHC on
bone marrow smears, sorry

Joyce Cline, H.T. (ASCP)
Technical Specialist
Hagerstown Medical Lab.
301-665-4980
fax 301-665-4941


-----Original Message-----
From: Rathborne, Toni [mailto:trathborne <@t> somerset-healthcare.com] 
Sent: Friday, November 14, 2008 1:30 PM
To: Joyce Cline
Subject: RE: [Histonet] Bone Marrow smears fixation

Joyce,
How long do you let the slides stay with the fumes? Have you run IHC on
slides fixed this way?

Thanks,
Toni

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Joyce
Cline
Sent: Friday, November 14, 2008 12:40 PM
To: Histonet
Subject: RE: [Histonet] Bone Marrow smears fixation


We give our Hematology department Superfrost+ slides to use for smears,
because we had the same problem. We also went back to the old fashioned way
of fixing the smears. We use a coplin jar with a small piece of papertowel
in the bottom and place a few drops of formalin in the coplin jar. The
smears are fixed in formalin fumes. We had a lot of smears that were not
staining for iron before using the methanol method.

Joyce Cline, H.T. (ASCP)
Technical Specialist
Hagerstown Medical Lab.
301-665-4980
fax 301-665-4941


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Georgine
Whitman
Sent: Friday, November 14, 2008 9:51 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Bone Marrow smears fixation

we are trying to do an iron  stain on  our bone marrow smears on the Ventana
Nexus. We are having trouble with the specimen staying on the slide. I
personally have not done iron on smears in years.  Our hematology department
has been doing them by hand and they are fixed in methanol alcohol.  
                                                          Please Help Thank
You Georgine
 



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