[Histonet] Buffer.
Tora Bardal
tora.bardal <@t> bio.ntnu.no
Thu Nov 13 13:34:59 CST 2008
Hello Ian,
We use a mixture of 2.5% glutardialdehyde, 2.5% paraformaldehyde and
0.5% sucrose in 0.08 M cacodylic buffer, pH 7.4 to 7.6
Galloway, Trina Falck; Kjørsvik, Elin; Kryvi, Harald.
Effect of temperature on viability and axial muscle development in
embryos and yolk sac larvae of the Northeast Arctic cod (*Gadus
morhua*).. /Marine Biology/ 1998 ;Volum 132.(4) s. 559-567
Tora
NTNU Center of Fisheries and Aquaculture
Ian Montgomery wrote:
> What is the recommended EM buffer for sea water fish? I had
>thought of using sea water itself but would cacodylate or one of the
>phosphates be better and at what molarity?
>
>Ian.
>
>
>
>
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