[Histonet] can anyone tell me...

[Kemlo Rogerson]

So it is 10% formol alcohol in 70% alcohol; wonder what the other 20% is? Water?

Alcoholic fixatives as a genre tend to overharden on standing but I guess fatty tissue would benefit. Why would anyone use something you don't actually know what it is composed of?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: 12 November 2008 13:48
To: rjbuesa <@t> yahoo.com; histonet <@t> lists.utsouthwestern.edu; Heath, Nancy L.
Subject: RE: [Histonet] can anyone tell me...

Found this on Histosearch.  Also, when I was in a hospital lab we used it all the time on breast and any fatty tissues with great success. 

http://www.histosearch.com/histonet/Jan00/Penfix.html


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, November 12, 2008 8:42 AM
To: histonet <@t> lists.utsouthwestern.edu; Heath, Nancy L.
Subject: Re: [Histonet] can anyone tell me...

Nancy:
Well this is a different question altogether, you are wondering about if you should use Penfix versus NBF (you should never use unbuffered 10% formalin).
Penfix is a commercial mixture of less than 10% formalin + methanol + ethanol + 2-propanol (3 different alcohols) in undisclosed (proprietary amounts) never evaluated independently (meaning that the only "evaluation" was done by the manufacturer).
It has been said (anecdotalal information) that "overfixes" and dries small biopsies, something understandable due to the alcohols it contains.
Since alcohols and formalin fix tissues in very different ways I personally do not see any advantage (and I could think on some disadvantages) of this "combined" fixation.
I personally would NOT use it. Now it is after you.René J.

--- On Wed, 11/12/08, Heath, Nancy L. <NHeath <@t> Lifespan.org> wrote:


From: Heath, Nancy L. <NHeath <@t> Lifespan.org>
Subject: [Histonet] can anyone tell me...
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, November 12, 2008, 6:40 AM

Hi Everyone :)
 
Thanks to all for answering my question about why you would put bone in PennFix before decal :) Maybe I should have written the question a bit differently. I know bone is to be well fixed before decal...I just wanted to know why someone would put bone in Pennfix versus 10% neutral buffered formalin??? Is there any difference or extra benefits with PennFix??
 
Thanks
 
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