[Histonet] can anyone tell me...

[Rene J Buesa]

Nancy:
Well this is a different question altogether, you are wondering about if you should use Penfix versus NBF (you should never use unbuffered 10% formalin).
Penfix is a commercial mixture of less than 10% formalin + methanol + ethanol + 2-propanol (3 different alcohols) in undisclosed (proprietary amounts) never evaluated independently (meaning that the only "evaluation" was done by the manufacturer).
It has been said (anecdotalal information) that "overfixes" and dries small biopsies, something understandable due to the alcohols it contains.
Since alcohols and formalin fix tissues in very different ways I personally do not see any advantage (and I could think on some disadvantages) of this "combined" fixation.
I personally would NOT use it. Now it is after you.René J.

--- On Wed, 11/12/08, Heath, Nancy L. <NHeath <@t> Lifespan.org> wrote:


From: Heath, Nancy L. <NHeath <@t> Lifespan.org>
Subject: [Histonet] can anyone tell me...
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, November 12, 2008, 6:40 AM

Hi Everyone :)
 
Thanks to all for answering my question about why you would put bone in
PennFix before decal :) Maybe I should have written the question a bit
differently. I know bone is to be well fixed before decal...I just
wanted to know why someone would put bone in Pennfix versus 10% neutral
buffered formalin??? Is there any difference or extra benefits with
PennFix??
 
Thanks
 
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