[Histonet] CELL CULTURE LINE ON SLIDES
lcrosby <@t> echelon-inc.com
Mon Nov 10 10:15:23 CST 2008
I routinely stain cells with fluorescent antibodies. I fix them with 4%
Paraformaldehyde in PBS, or the easier way, which I use now, is in neutral
buffered formalin, which is readily purchased and does not require the labor
intensive steps of the former.
Echelon Biosciences Inc.
801-588-0455 ext 329
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Monday, November 03, 2008 12:58 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] CELL CULTURE LINE ON SLIDES
We were asked to do immunostains on slides where a particular cell line was
growing. Can anyone tell me the best way to fix these slides before
performing immunostains? We used to use acetone on cytospins but can't
recall if there is a better way.
Jim Vickroy BS, HT(ASCP)
Technical Supervisor - Surgical and Autopsy Pathology
Memorial Medical Center
vickroy.jim <@t> mhsil.com
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