[Histonet] Trichrome Question
John Kiernan
jkiernan <@t> uwo.ca
Mon Nov 10 01:05:37 CST 2008
Dear Angela Fogg,<BR> <BR>It is difficult
to Gomori' published 20: 662-664 (1950)? Kiernan<BR>Anatomy, UWO<BR>Lon =<BR>----- Original Message -----<B ANGELAFOGG <@t> aol.com<BR>Date: Saturday, November 8 10:03<BR>Subject: Re: [Histonet] Trichrome Questi on<BR>To: jkiernan <@t> uwo.ca<BR><BR>>
<BR>&g <BR>> 6.00.6000.16735" name=GENERATOR> <TABLE> <TBODY> <TR> <TD id=role_body
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id=role_document face=Arial color=#000000 size= 2><BR>> Thanks for responding. Fixed in 10%
f alcoh <BR>> Surgipath. Cut at 5 microns Richard Allan's Chromaview Stain ki Trichrome. Used the <BR>> above meth routine Trichrome controls and all the muscle stained red as < BR>> they are supposed to be. That's why I put
ou the mu has <BR> well.</P><BR>> Thought ma formalin had something to do with the <BR> staining. Maybe longer than an hour in Bouin' continuing the <BR>> Trichrome would help?<BR> <BR>> Await your thoughts and info<BR><BR>>
Re < dated 11/8/2008 2: <BR>> jkiernan <@t> uw writes:<BR><BR>> style="PADDING-LEFT: 5px; MARGIN-LEFT: 5px; BO RDER-LEFT: blue 2px solid"><FONT
style="BACKGROUND-COLOR: color=#000000 size=2>Which <BR>& method? What kind of section (frozen, paraffin, pl astic?)<BR>> Any <BR>> post-fixation in
somethin <BR>> <BR> and you will get lots of advic <BR>> Weeks in "prolonged" fixation unless the <BR>> <BR>> are numerous enough to make years. As a genera lity, trichrome <BR>> methods <BR>> do not
work formaldeh Postfixation of th Bouin <BR>> is picric acid us <BR>> jus are<BR>> published reports that iodine and <BR>> buffer will improve trichrome<BR>&g staining of paraffin sections of <BR>> formaldehyde-fixe tissue. See Yu & <BR>> Chapman 2003 <EM>J.
<STRONG>26</STRONG>(2) <BR>> <BR>> "Trichrome" has been applied to several staini ng <BR>> techniques that use two <BR>> or more
dye trichrome met phosphomolybdic or phosphotun to <BR>> enable the sta cytoplasm by <BR>> anionic dye <BR>> contrasting colours: blue or <BR>> collagen, and red for cytoplasm<BR>&g (including smooth & striated muscle). A <BR>> third anionic dye, typically yellow <BR>> or orange,
may Instructions for < all <BR>> tex and<BR>> histotechnology. <BR>> John <BR>> Kiern an<BR>> Anatomy, UWO<BR>> London,
Canada<BR> Message <BR>&g angelafogg <@t> aol.com<BR>> 7, 2008 <BR>> 20:17<BR>& [Histonet] Trichrome Question<BR>> To: <BR>>
histonet <@t> lists.utsouthwestern.edu<BR>> <BR>> > <BR>> > Performed a
trichro <BR>& <BR>> Muscle st > red.?What happened? <BR>> fixation react this way?<BR>& Hope someone can shed some light on <BR>> this .<BR>> > Regards,<BR>> >
Angela< ___________ _______________________ 5F Histonet m Histonet <@t> lists. <BR>> http:// lists.utsouthwestern.edu/mailman/listinfo/histonet<BR>>
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