[Histonet] schiff's

[Joseph Saby]

I had a little problem once with Schiffs.

I was making up a new batch (I believe about 2 liters) and had just finished boiling the water.  I was in a hurry (often the culprit in disasterous lab events) and forgat that impure solvents have a lower boiuling point.  When I added the basic fuchsin, it erupted like a volcano!

Now, to get the full picture, you must envision a newly remodeled lab.  New tile floor.  Newly painted cupboards.  Fresh paint on the wall.  And, of course, the lab director a planning walk-through show-off tour of the new facility.

Bucket and mop to try to remove most of the excess.  Gallons of acid alcohol.  And, yes, 10% bleach solution for the fresh paint and the cabinets.  It took all day, but I got it looking good as new.

The next week, someone broke a container of eosin on the floor.  My boss forever blamed me for the orange-pink arising every time they mopped the floor...




________________________________
From: "renafail <@t> bellsouth.net" <renafail <@t> bellsouth.net>
To: "Swain, Frances L" <SwainFrancesL <@t> uams.edu>; Emily Sours <talulahgosh <@t> gmail.com>; histonet <@t> lists.utsouthwestern.edu
Sent: Friday, November 7, 2008 6:05:52 PM
Subject: RE: [Histonet] schiff's



A Liter!!! It took most of a day to clean 40 ml dropped in a dishwasher  to which the tech added  extra multiterge, which caused the dishwasher to overflow. A colorful event. My sympathy
Rena Fail
-------------- Original message from "Swain, Frances L" <SwainFrancesL <@t> uams.edu>: --------------


> If you have a staining problem from the spill a weak solution of bleach
> usually clears up any Schiff's that is dropped on the bench, floor, hands,
> etc.
> 
> Frances L. Swain HT(ASCP) A. A. S.
> Special Procedures Technician
> Department of Orthopaedic Surgery
> Center for Orthopaedic Research
> Barton Research Building 2R28
> 4301 West Markham Street
> Little Rock AR 72205
> (501) 686-8739 PHONE
> (501) 686-8987 FAX
> swainfrancesl <@t> uams.edu email
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily Sours
> Sent: Friday, November 07, 2008 1:12 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] schiff's
> 
> Schiff's reagent is my nemesis.  It's clear until it gets on anything
> outside of the bottle.
> Including the floor, clothing, and our fume hood.
> (Our post-doc had a little accident.  Actually a rather large one, involving
> 1 L of Schiff's.)
> 
> Emily
> 
> On Fri, Nov 7, 2008 at 1:42 PM, Charles, Roger  wrote:
> 
> > We are using Shiffs reagent.  When you put a drop of formalin in Shiffs
> > reagent it turns purple.
> >
> > Roger Charles
> > Microbiologist
> > Pennsylvania Veterinary Laboratory
> > 2305 N Cameron St
> > Harrisburg, PA 17110
> > 717-787-8808
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> > histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Smith Wanda
> > Sent: Friday, November 07, 2008 1:31 PM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] Is it Formalin-Without Smelling It?
> >
> > Happy Friday Everyone!!!!!
> > Silly question:  Is there an easy way to tell if a solution in a container
> > is/is not formalin without smelling it?????
> > Thanks,
> > Wanda
> >
> > WANDA G. SMITH, HTL(ASCP)HT
> > Pathology Supervisor
> > TRIDENT MEDICAL CENTER
> > 9330 Medical Plaza Drive
> > Charleston, SC  29406
> > 843-847-4586
> > 843-847-4296 fax
> >
> >
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> 
> 
> 
> -- 
> "You would know her for all the things she was...a woman who knew her way in
> and out of every new book without being singed, pinched, bumped or tickled
> by any line or chapter."
> John O'Hara, Appointment in Samarra
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