[Histonet] RE:CD4 on Ventana

[Milne, Katy]

Hi Piero, what clone are you using for CD4?

There seem to be 2 main clones, 1F6 or something like that and 4B12.
The epitope recognized by 1F6 doesn't do well in formalin so 4B12 is the
one most used.  Having said that, I've found it's still not wonderful, I
can get staining (I use a Ventana Discovery) but because the Ab is
recommended to be used at such a high concentration, you do have to pick
out the real staining against some background.

I used it in this paper, our general protocol is in there too but I'm
not sure how much the benchmark differs from the discovery....
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00034
09

Katy Milne 
Deeley Research Centre

Message: 19
Date: Fri, 7 Nov 2008 20:15:11 +1100
From: "Piero Nelva" <pieronelva01 <@t> bigpond.com>
Subject: [Histonet] CD4 on Ventana
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <2D0669F5E5EA4786A17AE07D652FFF51 <@t> pentium4>
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Dear Histonetters

I'm having a lot of trouble getting CD4 to stain FFPE tissue using the 
Ventana Benchmark XT.  Any advice about which antibody and protocol 
suggestions would be greatly appreciated.

Piero Nelva
Anatomical Pathology
Monash Medical Centre
Australia