[Histonet] CD4 on Ventana

[Sebree Linda A.]

Piero,

We use C4d from American Research Products, Inc., cat. #: 12-50000 at
1:50.  On the BenchMark XT we use mild CC1 for 32" at 42 degrees C with
AB block.

Hope this helps.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Piero
Nelva
Sent: Friday, November 07, 2008 3:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD4 on Ventana

Dear Histonetters

I'm having a lot of trouble getting CD4 to stain FFPE tissue using the 
Ventana Benchmark XT.  Any advice about which antibody and protocol 
suggestions would be greatly appreciated.

Piero Nelva
Anatomical Pathology
Monash Medical Centre
Australia 


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