[Histonet] stain

[Bryan Llewellyn]

Altmann's acid fuchsin picric acid is a histological stain for mitochondria.

It requres the tissue to be fixed in a non-acid dichromate fixative, then 
post-chromed for some time.  The tissue is then processed by paraffin and 
sectioned.

Staining is in aniline water saturated with acid fuchsin, which is applied 
much like carbol fuchsin in a ZN, i.e. heated.  Diffeerentiation is with 
picric acid.

The method requires considerable skill from the technologist, but produces 
red mitochondria on a yellow background.

I suggest that if you just want to demonstrate mitochondria, do a succinate 
dehydrogenase instead, it is much easier and much faster.

If you want, I can look up the details and send them on, but I recall they 
used to be in Culling's book.

Bryan Llewellyn


----- Original Message ----- 
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
To: <histonet <@t> pathology.swmed.edu>
Sent: Wednesday, November 05, 2008 2:27 PM
Subject: [Histonet] stain


> Anyone familiar with this stain?
>
>
>
> Altmann acid-fushsin picric. This stain is a histochemical stain for
> mitochondria in intestine.
>
>
>
> Best regards,
>
>
>
> Patsy
>
>
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email pruegg <@t> ihctech.net
> web site www.ihctech.net
>
>
>
>
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