[Histonet] 200 proof alcohol?

John C. Dennis dennijc <@t> vetmed.auburn.edu
Wed Nov 5 13:04:15 CST 2008


I would not recommend using the same alcohol for histo that you use for 
molecular work.  Assuming by "molecular" you mean isolating DNA/RNA, 
cloning, etc.  You'll want a higher grade, more expensive alcohol for 
nucleic acid work then the histological stuff that comes in a 55 gal drum.

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

On Wed, 5 Nov 2008, Caroline Bass wrote:

> Hello,
> I¹m trying to buy some alcohol for both histology and molecular biology
> protocols. But I¹m having some difficult getting ³molecular biology grade²
> 100% ethanol. The hospital I work at sells 200 proof ethanol, but can¹t say
> anything about the quality. Since it¹s 200 proof, is that basically the same
> as molecular biology grade? How about histology grade? I seem to recall
> using hospital provided ethanol for molecular biology experiments in my
> postdoc, so I think it should be ok.
> Any advice is appreciated!
> Caroline
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