[Histonet] wavy mid myocardium

[Geoff McAuliffe]

Hi Betsy:

    Wow, a real histology posting! If it is any comfort I have had the 
same problem with mouse heart after Zenker's fix (I wanted nice 
intercalated disks). I think the solution is to use NBF.

Geoff

Molinari, Betsy wrote:
> Hi,
>
> I processed some mouse hearts that were in Z-Fix for 24 hrs. then put in
> 70% for anywhere from 24-48hrs. They were processed on a short run. The
> epicardium  looks good but the mid myocardium is wavy.
>
> 15 min 80%
>
> 15 min 95 x 2
>
> 15 min 100% x 2
>
> 15 min Xylenes x2 
>
> 10 min paraffin x 4
>
> Any suggestions?
>
> Thanks.
>
>  
>
>  
>
> Betsy Molinari   HT(ASCP)
>
> Texas Heart Institute 
>
> Cardiovascular Pathology
>
> 6770 Bertner Ave.
>
> MC 1-283
>
> Houston, TX 77030
>
> 832-355-6524
>
> 832-355-6812 (fax)
>
>  
>
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>
>   


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff <@t> umdnj.edu
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