AW: [Histonet] double trouble

[Gudrun Lang]

We do an automated doublestain, that works with Peroxidase-DAB and Alk.
Phosphatase-Red. The critical point is, to perform a second HIER-step
between the two detection-sequences.
The fixed specimen don't suffer from the second heating, but the native
antibodies are destroyed and are not detectable with the
second-secondary-antibody. DAB sticks so well to the tissue, that it isn't
washed away by this treatment.

The nice think of this method is, that you don't have to think about
species.

Gudrun Lang



-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Edwards,
R.E.
Gesendet: Montag, 03. November 2008 15:09
An: histonet
Betreff: [Histonet] double trouble


OK chaps, which in your opinion is the  best double labelling method when
one  is  using primary antibodies raised in the  same  species, I am aware
that Vector labs supply such a kit; many  thanks.

                                      Cheers
                                             Richard Edwards
                                                        University of
Leicester

 
U.K....
                              

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